Gene Biotechnology

Covering state-of-the-art technologies and a broad range of practical applications, the Third Edition of Gene Biotechnology presents tools that researchers and students need to understand and apply today's biotechnology techniques. Many of the currently available books in molecular biology contain only protocol recipes, failing to explain the principles and concepts behind the methods outlined or to inform the reader of possible pitfalls in the methods described. Filling these gaps, this book: Discusses a wide variety of approaches, from very basic methods to the latest, most sophisticated technologies Contains clearly detailed, step-by-step protocols with helpful troubleshooting tips Addresses the needs of researchers in academic and commercial environments Guides graduate students in designing, implementing, and evaluating experimental projects. Each chapter covers the principles underlying methods and techniques, and includes step-by-step descriptions of each protocol, notes, tips, and a troubleshooting guide. The book includes sections on how to write a research paper for publication in English-language journals, how to protect research discoveries and inventions via patents, and practical methods of bio-calculation. Written by a team of internationally recognized scientists, Gene Biotechnology presents protocols as well as clear and simple explanations of the key principles and concepts behind the methods. It is a single, logically organized source forthe most important new methodologies. This unique resource provides the tools to help ensure success in contemporary molecular and cellular biology research.

Strategies for Novel Research Projects and Research Grant FundingRapid Isolation of Specific cDNA or Gene by PCRConstruction and Screening of Subtracted and Complete Expression cDNA LibrariesSubcloning of Genes or DNA FragmentsNon-isotopic and Isotopic DNA or RNA SequencingInformation Superhighway and Computer Databases of Nucleic Acids & ProteinsCharacterization of DNA or Genes by Southern Blot HybridizationGene Over-expression by Sense RNA in Mammalian SystemGene Under-expression by Antisense DNA and RNA StrategiesAnalysis of Gene Expression at the Functional Genomic Level Using Northern blotting and/or PCRAnalysis of Gene Expression at the Proteomic Level via Western BlottingAnalysis of Protein Expression Using Immuno-fluorescence LocalizationAnalysis of Cellular DNA or Abundance mRNA by Radioactivity In Situ Hybridization (RISH)Localization of DNA or Abundance mRNA by Fluorescence In Situ Hybridization (FISH)In Situ PCR Hybridization of Low Copy Gene and In Situ RT-PCRDetection of Low Abundance mRNAIsolation and Characterization of Genes from Genomic DNA LibrariesMouse Stem Cells as a Model Mammalian Cell line for Gene ExpressionNew Strategies for Gene KnockoutLarge-scale Expression and Purification of Recombinant ProteinsQuantitative Analysis of Functional Genome by Real-Time RT -PCRHigh-throughput Analysis of Gene Expression by DNA Microarrays (Gene Chips)Construction and Screening of Human Antibody Libraries Using Phage Display TechnologyDown-regulation of Gene Expression in Mammalian Systems via SiRNA TechnologyNew Techniques for Identification of Protein Binding and Proteomic Interactions

William Wu, Ph.D., has extensive experience in both academic and industrial settings. He held various positions, including Research Assistant Professor at the University of Michigan Medical School, Ann Arbor, Michigan, USA, and Professor at the Hunan Nonnal University, Hunan, the People's Republic of China, Senior Scientist, Team-Leader and Chief Technology Officer (CTO) in biomedical and pharmaceutical companies. Helen H. Zhang, MS., is Senior Research Associate at the University of Michigan Medical School, Ann Arbor, Michigan, USA. In 1996, she received her MS degree in Molecular and Cellular Biology at the Eastern Michigan University, Ypsilanti, Michigan, USA. Michael J. Welsh, Ph.D., is Professor of Cell Biology in the Department of Cell and Developmental Biology at the University of Michigan Medical School, Ann Arbor, Michigan, USA. He is also a Professor of Toxicology in the School of Public Health at the University of Michigan, Ann Arbor. Peter B. Kanfman, Ph.D., is Professor of Biology in the Plant Cellular and Molecular Biology Program in the Department of Biology and a member of the faculty of the Bioengineering Program at the University of Michigan.