Gamete Assessment, Selection and Micromanipulation in ART A Practical Guide

Part I: Human Oocyte Evaluation in IVF.- Assessment of Oocyte Quality.- Polarization Microscopy.- Cumulus Cell Gene Expression in Assessment of Oocyte Quality.- Part II: Sperm Evaluation and Selection.- Sperm Assessment: Traditional Approaches and Their Indicative Value.- Sperm Assessment: Novel Approaches and Their Indicative Value.- Intracytoplasmic Morphologically Selected Sperm Injection.- Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in Andrology and IVF Laboratories.- Electrophoretic Sperm Separation.- Magnetic-Activated Cell Sorting of Human Spermatozoa.- Polscope-Based Sperm Selection.- Part III. Micromanipulators and Micromanipulation.- Hydraulic Manipulators for ICSI.- Research Instruments Micromanipulators.- Eppendorf Micromanipulator: Setting Up and Operation of Micromanipulators.- The Leica Microsystem IMSI System.- Automated Robotic Intracytoplasmic Sperm Injection.- Oocyte Treatment and Preparation for Microinjection.- Mechanism of Human Oocyte Activation during ICSI and Methodology for Overcoming Low or Failed Fertilization.- Livestock Production via Micromanipulation.- Assisted Hatching in IVF.- Part IV: Biopsy Procedures on Oocytes and Embryos.- Polar Body Biopsy.- Cleavage-Stage Embryo Biopsy.- Embryo Biopsy for PGD: Current Perspective.- Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects.- Part V: Molecular Insights.- The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development.- Nuclear and Cytoplasmic Transfer: Human Applications and Concerns.- Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization.- Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence.