Forensic and Clinical Applications of Solid Phase Extraction

This complete laboratory reference manual explains the principles behind solid phase extraction (SPE) and provides readily reproducible protocols for solving extraction problems in forensic and clinical chemistry. Numerous actual chromatograms, based on original research and diverse applications, demonstrate the technique and the results that can be achieved. Extensive appendices allow fast access to frequently needed information on reagents, the preparation of solutions and buffers, milliequivalent and millimode calculations, buffers and pKa for SPE, and a complete RapidTrace® technical manual. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and on avoiding known pitfalls.

1: Introduction to Solid Phase Extraction.- 2: Silica-Based Solid Phase Extraction.- 3: The Chemistry of Bonded Silicas.- 4: Bonded Phase Sorbent Synthesis.- 5: Strategies for the Use of Solid Phase Extraction.- 6: Optimizing Solid Phase Extraction Methods.- 7: Separation of Analytes from Their Matrices.- 8: Solid Phase Extraction Troubleshooting.- 9: Drug Methods for the Toxicology Lab.- 10: Selected Solid Phase Extraction Applications in Drug Analysis.- 11: General Methods for Drug Isolation.- 12: Chemical Derivatization.- Appendix A: Glossary.- Appendix B: Reagents.- Appendix C: Preparation of Solutions and Buffers.- Appendix D: Milliequivalent and Millimole Calculations.- Appendix E: Selected Solvent Polarity and Property Scale.- Appendix F: Buffers for Solid Phase Extraction.- Appendix I: Further Reading.