Familial Cardiomyopathies Methods and Protocols

This volume covers the latest advances in technologies that look at familial cardiomyopathies in greater detail, and provides new computational and experimental models that model, study, and detect disease at earlier stages. Together, this allows interdisciplinary research experiments to provide new insights for the development of novel interventions that slow, stop, or even reverse the disease process. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and practical, Familial Cardiomyopathies: Methods and Protocols aims to inspire further development of techniques used to study myocardial disease and the development of new, sarcomere-targeted therapeutic approaches for the maintenance of heart health and the treatment of heart failure.

Echocardiography to Assess Cardiac Structure and Function in Genetic Cardiomyopathies.- MRI Quantification of Cardiac Structure and Function in Cardiomyopathy Patients.- Atomistic Simulations of Sarcomere Proteins.- Design Principles and Benefits of Spatially Explicit Models of Myofilament Function.- Computational Modeling of Cardiac Electrophysiology.- Computational Modeling and Simulation of the Fibrotic Human Atria.- A Semi-Automatic Pipeline for Generation of Large Cohorts of Four-Chamber Heart Meshes.- Genomic Engineering of Induced Pluripotent Stem Cell-Derived Cardiomyocytes.- The GENTIL Method for Isolation of Human Adult Cardiomyocytes from Cryopreserved Tissue for Proteomic Analyses.- Motility Assay to Probe the Calcium Sensitivity of Myosin and Regulated Thin Filaments.- Protocols for Myosin and Actin-Myosin Assays using Rapid, Stopped-Flow Kinetics.- Measuring the Contractile Kinetics of Isolated Myofibrils from Human-Induced Pluripotent Stem Cell Derived Cardiomyocyte (hiPSC-CM) Models of Cardiomyopathy.