Experimental Models of Cardiovascular Diseases Methods and Protocols

This book provides methodological information on establishing reliable and reproducible experimental models of cardiovascular diseases. Chapters detail practical protocols from expert laboratories focusing on cardiovascular research, leading to novel discoveries in cardiac biology,  and the development of effective therapeutic approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

 Authoritative and cutting-edge, Experimental Models of Cardiovascular Disease: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Experimental Models of Cardiovascular Diseases - Overview -.- An Introduction to Computational Modeling of Cardiac Electrophysiology and Arrhythmogenicity.- Isolation of Atrial and Ventricular Cardiomyocytes for In Vitro Studies.- Cardiomyocyte Differentiation from mouse Embryonic Stem Cells.- Cardiomyocyte Differentiation from Human Embryonic Stem Cells.- Induction of Human Induced Pluripotent Stem Cells to Cardiomyocytes using Embryoid Bodies.- Measuring Cardiomyocyte Contractility and Calcium Handling In Vitro.- Langendorff Perfusion Method as an Ex Vivo Model to Evaluate Heart Function in Rats.- Methods for the Preparation of an Excised, Cross-circulated Rat Heart.- Optical Action Potential Mapping in Acute Models of Ischemia-reperfusion Injury: Probing the Arrhythmogenic Role of the Mitochondrial Translocator Protein.- Cardiac Tissue Engineering Models of Inherited and Acquired Cardiomyopathies.- Badimon Perfusion Chamber: An Ex Vivo Model of Thrombosis.- Ischemic Model of Heart Failure in Rats and Mice.- Conventional Method of Transverse Aortic Constriction in Mice.- Characterization of the Differential Progression of Left Ventricular Remodeling in a Rat Model of Pressure Overload Induced Heart Failure. Does Clip Size Matter?.- Isoproterenol-induced Heart Failure Mouse Model Using Osmotic Pump Implantation.- Rat model of Cardiotoxic Drug Induced Cardiomyopathy.- Pulmonary Artery Hypertension Model in Rats by Monocrotaline Administration.- The Sugen 5416/Hypoxia Mouse Model of Pulmonary Arterial Hypertension.- Mouse Model of Wire Injury-Induced Vascular Remodeling.- The Mouse Aortocaval Fistula Model with Intraluminal Drug Delivery.- A Pig Model Of Myocardial Infarction: Catheter Based Approaches.- Ovine Model of Ischemic Mitral Regurgitation.- Canine Model of Pacing-induced Heart Failure.- Swine Model of Mitral Regurgitation Induced Heart Failure.- Pig Model of Increased Cardiac Afterload Induced by Ascending Aortic Banding.- Large Porcine Model of Profound Acute Ischemic Cardiogenic Shock.- Chronic Pulmonary Artery Embolization Models in Large Animals.- Modeling Pulmonary Hypertension: A Pig Model Of Post-capillary Pulmonary HypertensionDevelopment and Multiparametric Evaluation of Experimental Atherosclerosis in Rabbits.