DNA Electrophoresis Methods and Protocols

 Introduction and Perspectives of DNA Electrophoresis.- Two-Dimensional Gel Electrophoresis to Resolve DNA Topoisomers.- Using Two-Dimensional Intact Mitochondrial DNA (mtDNA) Agarose Gel Electrophoresis (2D-IMAGE) to Detect Changes in Topology Associated with Mitochondrial Replication, Transcription, and Damage.- 2D Gel Electrophoresis to Detect DNA Replication and Recombination Intermediates in Budding Yeast.- Neutral-Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures.- Alkali Comet Assay in Genotoxicity Tests.- The Analysis of DNA Interstrand Crosslinks and their Repair by the Modified Comet Assay.- Analysis of Chromosomal DNA Fragmentation in Apoptosis by Pulsed-Field Gel Electrophoresis.- Detection of DNA Damage-Induced DSBs by the Contour-Clamped Homogeneous Electric Field (CHEF) System in Mammalian Cells.- Investigation of DNA Double-Strand Breaks Induced in Host Cells following Infection with Genotoxic Bacteria.- Monitoring of DNA Replication and DNA Double-Strand Breaks in Saccharomyces cerevisiae by Pulsed-Field Gel Electrophoresis (PFGE).- Pulsed-Field Gel Electrophoresis for Detecting Chromosomal DNA Breakage in Fission Yeast.- Detection of DNA Double-Strand Breaks by Pulsed-Field Gel Electrophoresis of Circular Bacterial Chromosomes.- Detection of Bleomycin-Induced DNA Double-Strand Breaks in Escherichia coli by Pulsed-Field Gel Electrophoresis using a Rotating Gel Electrophoresis System.- Circle-Seq: Isolation and Sequencing of Chromosome-Derived Circular DNA Elements in Cells.- Chromatin Pull-Down Methodology Based on DNA Triple Helix Formation.- DNA Fragments Agarose Gel Electrophoresis for Chromatin Immunoprecipitation (ChIP).- Post-Labeling/PAGE Method for Detection of DNA Adducts.