Clinical Applications of Mass Spectrometry in Drug Analysis Methods and Protocols

This fully updated volume describes methods and protocols for a number of drugs and toxins in a stepwise manner. Exploring the versatility and flexibility of mass spectrometry, the book covers the advantages of this technology, which typically include elimination of the need for special reagents such as antibodies, increased sensitivity and specificity, and multi-component analysis enabling the screening of tens to hundreds of compounds in a single assay run. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. 

Mass Spectrometry in Clinical Laboratory: Applications in Therapeutic Drug Monitoring and Toxicology.- Quantitative LC-MS/MS Analysis of Amphetamines and Cocaine in Human Urine.- Quantification of Gabapentin, Lacosamide, Perampanel, Pregabalin, Rufinamide, and Vigabatrin in Serum Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Turbulent Flow Liquid Chromatography-Tandem Mass Spectrometry Methods for Antiepileptic Drug Quantitation in Serum.- Quantitative LC-MS/MS Method for the Simultaneous Measurement of Six Antiepileptics and Pentobarbital in Human Serum.- Triazole Antifungal Quantification for Therapeutic Drug Monitoring in Serum by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS):  Posaconazole, Voriconazole, Itraconazole, and Hydroxyitraconazole.- Liquid Chromatography-Tandem Mass Spectrometry-Based Therapeutic Monitoring of Plasma Atovaquone Concentrations in Pediatric Patients.- Analysis of Barbiturates in Urine by LC-MS/MS.- High Throughput Quantitative LC-MS/MS Analysis of Barbiturates in Human Urine.- High Throughput Quantitative LC-MS/MS Analysis of Benzodiazepines in Human Urine.- Quantitation of Bupropion in Urine by Liquid Chromatography-Tandem Mass Spectrometry.- Quantitative Analysis of Buprenorphine, Norbuprenorphine, and Their Glucuronide Metabolites in Human Urine.- Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of Plasma Busulfan.- Determination of Busulfan and Melphalan in Plasma by Turbulent Flow Chromatography-Tandem Mass Spectrometry.- Serum and Urine Quantification of Delta-8 and Delta-9 Tetrahydrocannabinol Carboxylic Acid Using Gas Chromatography-Mass Spectrometry.- 11-Nor-9-Carboxy-?8-Tetrahydrocannabinol, 7-Carboxy Cannabidiol, and 11-Nor-9-Carboxy-?9-Tetrahydrocannabinol in Urine by LC-MS/MS.- Quantification of Clofarabine and Fludarabine in Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry.- Untargeted High-Resolution Mass Spectrometry for Detection of Natural Products and Confiscated Powders and Pills.- Drug Screening of 49 Compounds by Liquid Chromatography-Tandem Mass Spectrometry in Saliva.- High Throughput Comprehensive Quantitative LC-MS/MS Analysis of a Common Drugs and Metabolites (62 Compounds) in Human Urine.- Detection of 41 Drugs and Metabolites in Urine with Internal Hydrolysis Control by Liquid Chromatography-Tandem Mass Spectrometry.- Comprehensive Urine Drug Screen by Gas Chromatography-Mass Spectrometry (GC-MS).- Comprehensive Urine Drug Screen by Liquid Chromatography-Quadrupole Time of Flight Mass Spectrometry.- A Liquid Chromatography-Tandem Mass Spectrometry Assay for Quantification of Ethyl Glucuronide and Ethyl Sulfate in Urine.- Quantification of Fentanyl and Norfentanyl in Whole Blood Using Liquid Chromatography-Tandem Mass Spectrometry.- Quantitative Assay of Fentanyl and Analogues Using LC-MS/MS.- Analysis of Hypoglycemic Drugs by Liquid Chromatography-Tandem Mass Spectrometry.- Multiplex Immunosuppressant (ISD) Method for the Measurement of Cyclosporine A, Tacrolimus, Sirolimus/Rapamycin, and Everolimus in Whole Blood Using MassTrak^TM Kit.- Assessment of Iohexol Serum Clearance by LC-MS/MS with Isotopically Labeled Internal Standard.- Quantification of Mycophenolic Acid in Plasma by High Performance Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantitation of Urine Nicotine, Cotinine, and 3-OH-Cotinine by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantification of Olanzapine by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantitative Opiate, Opioid, and Benzodiazepine Testing by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Quantitative LC-MS/MS Analysis of Opiates and Opioids in Human Urine.- Quantification of Oxcarbazepine Using Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Liquid Chromatography-Tandem Mass Spectrometry for Quantitation of Serum Pentobarbital.- Quantification of Phencyclidine (PCP) in Urine, Serum, or Plasma by Gas Chromatography-Mass Spectrometry (GC-MS).- Simultaneous Determination of Prednisone and Prednisolone in Serum by Turbulent Flow Liquid Chromatography-Tandem Mass Spectrometry.- Measurement of ThioTEPA and Its Metabolite TEPA in Plasma and Cerebrospinal Fluid by Turbulent Flow Chromatography-Tandem Mass Spectrometry.- A Direct Dilute and Shoot HPLC-MS/MS Method for Tetrahydrocannabinol (THC) Metabolites.- Quantification of Thiopurine Metabolites in Human Erythrocytes by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).- Determination of Treosulfan and Fludarabine in Plasma by Turbulent Flow Liquid Chromatography-Tandem Mass Spectrometry (TFLC-MS/MS).- Determination of Thiopurine S-Methyltransferase (TPMT) Activity in Human Erythrocytes by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).