Cereal Genomics Methods and Protocols

In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

 1. Dna Extraction from Vegetative Tissue for Next Generation Sequencing

Agnelo Furtado

2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)

Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa

3. RNA Extraction from Cereal Vegetative Tissue

Julie A. Pattemore

4. RNA Extraction from Developing or Mature Wheat Seeds

Agnelo Furtado

5. cDNA Library Preparation

Maarten Kooiker and Gang-Ping Xue

6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants

Siddanagouda S. Biradar, Xiaojun Nie, Kewei Feng and Song Weining

7. The Polymerase Chain Reaction (PCR) - General Methods

Daniel L. E. Waters and Frances M. Shapter

8. Mutation and Mutation Screening

L. Slade Lee, Bradley J. Till,  Helen Hill, Owen A. Huynh, and Joanna Jankowicz-Cieslak

9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression

Timothy L. Fitzgerald and Richard B. McQualter

10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel 

Agnelo Furtado

11. Rapid Cloning of Genes and Promoters for Functional Analyses

Peer M. Schenk

12. Genome Walking

Frances M. Shapter and Daniel L.E. Waters

13. Functional Analysis by Protein Biochemistry

Louis M. T. Bradbury

14. Genomic Southern Blot Analysis

Leigh Gebbie

15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs

Shazia Iram

16. Protein Blotting-Protocol for Beginners

Lars A. Petrasovits

17. Genetic Transformation of Wheat via Particle Bombardment

Caroline A. Sparks & Huw D. Jones

18. Sorghum Genetic Transformation by Particle Bombardment

Guoquan Liu, Bradley C. Campbell and Ian D. Godwin

19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery

Caroline A. Sparks, Angela Doherty & Huw D. Jones

20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation

Wendy A Harwood

21. Agrobacterium-mediated Transformation- Rice Transformation

Inez H. Slamet-Loedin, Prabhjit Chadha-Mohanty, Lina Torrizo

22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos

Hyeyoung Lee and Zhanyuan J. Zhang

23. A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops

Zining Wang

24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data

Gopala Krishnan, S., Daniel L. E. Waters and Robert J. Henry