Cellular Programming and Reprogramming Methods and Protocols

Before the therapeutic potential of cell replacement therapy or the development of therapeutic drugs for stimulating the body’s own regenerative ability to repair cells damaged by disease and injury can be fully realized, control of stem cell fate, immuno-rejection, and limited cell sources must be overcome. In Cellular Programming and Reprogramming: Methods and Protocols, expert researchers cover the most recent technologies and their related mechanisms involved in the programming and reprogramming of cell fate. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols, and notes to highlight tips on troubleshooting and avoiding known pitfalls.

Essential and cutting-edge, Cellular Programming and Reprogramming: Methods and Protocols promises to aid scientists attempting to advance stem cell biology in order to better treat devastating human diseases, including cardiovascular disease, neurodegenerative disease, musculoskeletal disease, diabetes, and cancer.

Human Embryonic Stem Cell Derivation, Maintenance, and Differentiation to Trophoblast.- Isolation and Maintenance of Mouse Epiblast Stem Cells.- Functional Assays for Hematopoietic Stem Cell Self-Renewal.- Isolation Procedure and Characterization of Multipotent Adult Progenitor Cells from Rat Bone Marrow.- Generation of Functional Insulin-Producing Cells from Human Embryonic Stem Cells In Vitro.- Mesoderm Cell Development from ES Cells.- Directed Differentiation of Red Blood Cells from Human Embryonic Stem Cells.- Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs.- Directing Human Embryonic Stem Cells to a Retinal Fate.- Bovine Somatic Cell Nuclear Transfer.- Cell Fusion-Induced Reprogramming.- An Improved Method for Generating and Identifying Human Induced Pluripotent Stem Cells.- Using Small Molecules to Improve Generation of Induced Pluripotent Stem Cells from Somatic Cells.- Reprogramming of Committed Lymphoid Cells by Enforced Transcription Factor Expression.- Reprogramming of B Cells.- Adult Cell Fate Reprogramming: Converting Liver to Pancreas.- In Vitro Reprogramming of Pancreatic Cells to Hepatocytes.- Generation of Novel Rat and Human Pluripotent Stem Cells by Reprogramming and Chemical Approaches.- Small Molecule Screen in Zebrafish and HSC Expansion.- Zebrafish Small Molecule Screen in Reprogramming/Cell Fate Modulation.