Cell Senescence Methods and Protocols

 Cell senescence is the process whereby cells permanently lose the possibility to proliferate without undergoing cell death, and occurs in a plethora of distinct model organisms. In Cell Senescence: Methods and Protocols, expert researchers in the field detail the methods that are now commonly used to study cell senescence, in model organisms encompassing bacteria, fungi, worms, flies, zebrafish, and mammalian cells. These techniques cover the study of all the morphological, biochemical and functional manifestations of senescence at the cellular level and include protocols for population analyses and high-throughput approaches in suitable model organisms. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Cell Senescence as Both a Dynamic and a Static Phenotype.-Senescence Regulation by mTOR.-Senescence Regulation by the p53 Protein Family.-Markers of Cellular Senescence.-Biomarkers of Cell Senescence Assessed by Imaging Cytometry.-Cytofluorometric Assessment of Cell Cycle Progression.-Quantification of Cell Cycle-arresting Proteins.-Colorimetric Detection of Senescence-associated ß Galactosidase (SA-ßgal).-Chemiluminescent Detection of Senescence-Associated ß Galactosidase (SA-ßgal).-Detection of the Senescence-associated Secretory Phenotype (SASP).-Unbiased Characterization of the Senescence-associated Secretome using SILAC-Based Quantitative Proteomics.-Detection of Senescence-associated Heterochromatin Foci (SAHF).-Monitoring DNA Damage During Cell Senescence.-Assessment and Quantification of Telomerase Enzyme Activity.-Methods for the Assessment of Telomere Status.-Detection of Nuclear Envelope Alterations in Senescence.-Measuring Reactive Oxygen Species in Senescent Cells.-Quantification of Protein Carbonylation.-Assays for the Measurement of Lipid Peroxidation.-Raman Spectroscopy for the Detection of AGEs/ALEs.-Monitoring Oncogenic B-RAF-induced Senescence in Melanocytes.-Methods to Investigate the Role of SIRT1 in Endothelial Senescence.-Monitoring Nutrient Signaling Through the Longevity Protein p66^SHC1.-Profiling the Metabolic Signature of Senescence.-Genome-wide RNAi Screening to Identify Regulators of Oncogene-induced Cellular Senescence.-An Integrated Approach for Monitoring Cell Senescence.-Robust Multiparametric Assessment of Cellular Senescence.-Assessing Chronological Aging in Bacteria.-Assessing Organismal Aging in the Filamentous Fungus Podospora anserina.-Assessing Chronological Aging in Saccharomyces cerevisiae.-Assessing Aging and Senescent  Decline in Caenorhabditis elegans: Cohort Survival Analysis.-High Throughput and Longitudinal Analysis of Aging and Senescent Decline in Caenorhabditiselegans.-Assessing Senescence in Drosophila using Video Tracking.-Assessing Vascular Senescence in Zebrafish.