Cell Cycle Oscillators Methods and Protocols

This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.

Part I Reviews

1. Cell Cycle Control: A System of Interlinking Oscillators

Randy Y.C. Poon

 

2. Model Organisms for Studying the Cell Cycle

Zhaohua Tang

 

3. Role of Computational Modelling in Understanding Cell Cycle Oscillators

Attila Csikász-Nagy and Ivan Mura

 

4. E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene Expression

Ingrid Thurlings and Alain de Bruin

 

Part II Methods

5. Cell Synchronization of Mouse Embryonic Fibroblasts

Michael J. Thwaites, Courtney H. Coschi, Christian E. Isaac, and Frederick A. Dick

 

6. Cell Cycle Synchronisation in Xenopus Egg Extracts

Peter J. Gillespie, Julia Neusiedler, Kevin Creavin, Gaganmeet Singh Chadha,  and J. Julian Blow

 

7. Elutriation for Cell Cycle Synchronization in Fission Yeast

Kazunori Kume

 

8. Spatiotemporal Investigation of Phosphorylation Events during Cell Cycle Progression

Lilia Gheghiani and Olivier Gavet

 

9. Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative Immunofluorescence

Karen Akopyan, Arne Lindqvist, and Erik Müllers

 

10. Building a Synthetic Transcriptional Oscillator

Matthaeus Schwarz-Schilling, Jongmin Kim, Christian Cuba, Maximilian Weitz, Elisa Franco, Friedrich C. Simmel

 

11. Time Series Simulations and Bifurcation Analyses of a Model of Mitotic Oscillations in Xenopus Cell-free Extracts

Tongli Zhang

 12. A Computational Method for Identifying Yeast Cell Cycle Transcription Factors

Wei-Sheng Wu

 

13. Measuring Activity and Specificity of Protein Phosphatases

Brendan L. Powers, Michael Melesse, Christie L. Eissler, Harry Charbonneau, and Mark C. Hall

 

14. Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an in vitro Cdk1 Targeting Assay to Study the Regulation of the g-Tubulin Complex

Po Hien Ear, Jacqueline Kowarzyk , Michael J. Booth, Diala Abd-Rabbo, Kristian Shulist, Conrad Hall, Jackie Vogel, and Stephen W. Michnick

 

15. Cell Cycle Synchronisation Using a Microfluidic Synchroniser for Fission Yeast

Cells

Shujing Wang and Chunxiong Luo

 

16. Detection of Protein-Protein Interactions in Tobacco BY-2 cells Using BimolecularFluorescence Complementation

Gemma S. Puts and Natasha Spadafora

 

17. Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic Bypass

Victoria Griesdoorn^, M Rowan Brown, Marie Wiltshire, Paul J Smith, and Rachel J Errington

 

18. Measuring APC/C Dependent Ubiquitylation in vitro

Marc A. Jarvis, Nicholas G. Brown, Edmond R. Watson, Ryan VanderLinden, Brenda A. Schulman, and Jan-Michael Peters

 

19. Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila Cells

Zielke, N., van Straaten, M. and Edgar, BA

 

20. Imaging Cell Cycle Phases and Transitions of Living Cells From Yeast to Woman

Hadas Segev, Drora Zenvirth, Kobi J. Simpson-Lavy, Naomi Melamed-Book,  and Michael Brandeis

 

21. Measurement of Cdk1/cyclin B Kinase Activity by Specific Antibodies and Western Blotting

Cody W. Lewis, Ryan G. Taylor, and Roy M. Golsteyn

 

22. The use of SNAP Labeling to Study Cell Cycle Oscillatory Proteins

Christine Greil, Marie Follo, Monika Engelhardt, and Ralph Wäsch