Cancer Cell Dormancy Methods and Protocols

This volume provides protocols and techniques to study metastatic dormant cells. Chapters focus on in vitro systems to model metastatic dormancy in vitro, detection of disseminated tumor cells in vivo, perturbation of key molecules involved in metastatic dormancy,  and clinical features of delayed metastatic relapses of specific tumor types. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Cancer Cell Dormancy: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Clinical Tumor Dormancy.- In vitro and in vivo systems to study tumor dormancy and the transition to overt metastases induced by the fibrotic milieu.- Modeling tumor cell dormancy in an ex vivo liver metastatic niche.- Bone marrow stoma co-cultivation model of breast cancer dormancy.- Establishing and characterizing chemotherapy-induced immunological dormant tumor cell lines.- In ovo xenograft model to study tumor dormancy, tumor cell dissemination and metastasis.- Pre-clinical mouse intraductal model (MIND) to study metastatic dormancy in estrogen receptor-positive breast cancer.- Intracardiac injection mouse model to study cancer cell dormancy in brain metastasis.- Modeling the depth of cellular dormancy from RNA-sequencing data.- Crispr knock-ins in organoids to track tumor cell subpopulations.- A live tracker of dormant disseminated tumor cells.- Characterizing rare dormant and cycling lineages using thewatermelon system.- Kiss1 and kisspeptins detection in cell lines.- Detection of ROS in dormant breast cancer cell.-13C tracer analysis and metabolomics in dormant cancer cells.- Visualizing the cell-matrix interactions and cytoskeleton of disseminated tumor cells.