Bunyaviruses Methods and Protocols

This volume explores the latest advancements in the study of bunyaviruses. The chapters in this book cover topics such as virus manipulation and purification; expression of recombinant proteins; the experimental study of bunyavirus infection in animals for virological and immunological analysis; and plant bunyaviruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on trouble shooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Bunyaviruses: Methods and Protocols is a valuable resource for both expert and novice scientists who are interested in learning more about this important and developing field.

An Introduction to the Class Bunyaviricetes.- Use of Rift Valley Fever Virus Expressing NanoLuc Luciferase for the Assessment of Neutralizing Antibodies and Antivirals.- A Serological Assay for the Detection of Antibodies Generated Against California Serogroup Virus Infection.- Molecular Diagnosis of Phlebovirus riftense Infection Using an L-Segment Based Real-Time RT-PCR Method.- Ultracentrifugation Purification of Cache Valley Virus Using Iodixanol.- Cryogenic Electron Microscopy of Rift Valley Fever Virus.- Detection and Characterization of Antiviral Proteins against Bunyaviruses in Mosquito-Derived Cells.- Vector Competence Assays for RVFV in Mosquitoes.- Experimental Infection of Ticks with SFTSV.- Methods for Thrips Transmission and Maintenance of Tomato Spotted Wilt Virus (TSWV).- In Vitro Evaluation of Bunyavirus T-Cell Immunity.- Analysis of Leishbuviridae from Trypanosomatids.- Recombinant Protein Production and Purification of Rift Valley Fever Virus Nucleoprotein from Escherichia coli Expression Systems.- Bunyaviruses: Expression of Recombinant Gycoproteins from Insect Cells.- Murine Models to Study Crimean-Congo Hemorrhagic Fever Hepatic Injury.- A Review on Reliable and Standardized Animal Models to Study the Pathogenesis of Schmallenberg Virus in Ruminant Natural Host Species.- Schmallenberg Virus, From the Necropsy Room to the Laboratory.- Reverse Genetics System for Crimean-Congo Hemorrhagic Fever Virus.- Recombinant Vaccine Production: Production of a Recombinant CCHF MVA Vaccine.- Efficient Expression of Oropouche Virus Nonstructural Proteins NSs and Nsm.- Systemic Clodronate Liposome Treatment for Depletion of Blood-Contacting Phagocytes and Investigation of Their Role in the Control of Bunyavirus Viremia.