Biochemical Engineering for 2001 Proceedings of Asia-Pacific Biochemical Engineering Conference 1992

Biochemical engineering forms a bridge between fundamental biochemical research and large scale biotechnology processes. It covers genetic and protein engineering, cell culture, bioprocess and reactor design, separation and modelling. Research work in biochemical engineering is an investment in the future, when conventional resources will have to be replaced with renewable ones. In this book the papers presented at the Asia-Pacific Biochemical Engineering Conference (Yokohama, Japan 1992) are collected. This collection is unique in its wide coverage of topics and it gives an overview of the current trends of research in an important area.

1. Overview.- Biochemical Engineering — Past, Present and Future.- Strategic Planning and New Directions in Biochemical Engineering.- New Biocatalytic Functions of Microorganisms and Their Industrial Applications.- 2. Gene and Protein Engineering.- Development of a Bacillus brevis Host-Vector System for Efficient Heterologous Protein Production and Its Application to Protein Engineering.- Chimeric Receptor Made from IgM and EGFR that Induces Phosphorylation Upon Binding with Antigen.- Molecular Cloning of Feline Interferon cDNA by Direct Expression and Its Mass Production Using Silkworm.- Expression of Human Calcitonin in Escherichia coli.- Control of Gene Expression and Overproduction of Useful Heterologous Protein by Using an On-Line Sensor.- Expression and Secretion of Cloned Proteins in Recombinant Saccharomyces cerevisiae.- Plasmid Loss Probability in Escherichia coli as a Function of the Growth Rate of Plasmid-Bearing Cells. I: Variation During Continuous Cultures.- Plasmid Loss Probability in Escherichia coli as a Function of the Growth Rate of Plasmid-Bearing Cells. II: Modelling for Continuous Cultures.- Cloning and Expression of the Xylose (Glucose) Isomerase Gene from the Thermophile Thermus thermophilus HB8.- Mutagenesis Studies on the Amino Acid Residues Involved in an Iron-Binding Domain and a Catalytic Site of Human 5-Lipoxygenase.- Technology of Production of Recombinant Prourokinase.- High Level Expression and Secretion of Biologically Active Leech Hirudin Variant 1 (HV1) by Escherichia coli.- Transfer of Isolated Nuclei of Saccharomyces cerevisiae Into Anucleated Protoplasts of Nectaromyces species.- 3. Enzyme Engineering.- In Vitro Protein Refolding.- Nitrile Hydratase: Investigation of Its Light-Induced Activation.- Cloning of Bacillus licheniformis Amylase Gene and Its Application to Synthesis of Branched Oligosaccharides.- Aldolases for Use in Carbohydrate Synthesis : Enzymatic Reaction Engineering as a Tool for Process Optimization.- Bioconversion of Gaseous Substrate by Methylotrophic Hansenula polymorpha in a Gas-Solid Bioreactor.- Asymmetric Hydrolysis of Phenylglycidate Ester by Esterase from Serratia marcescens.- D-Threonine Aldolase and Its Application to D-ß-Hydroxy-a-Amino Acid Synthesis.- Improvement of Enzymatic Properties by Hydrophobic Interaction.- The Effect of Amphopathics on the Properties of Enzymes (Lipase, Cholinesterase, Cellulase).- Modified Cellulase with Amphiphilic Copolymers: Solubilization in Organic Solvent.- Aminoacylase-Catalyzed Synthesis of N-Acyl Amino Acid from Fatty Acid or Its Ethyl Ester and Amino Acid.- Peptide Synthesis in Organic Solvent with an Immobilized Enzyme.- A Novel Interesterification Process for Fats and Oils Using Acetone-Dried Fungus Immobilized in Biomass Support Particles.- Fractionation of Cellulase Components by Affinity Precipitation: Production of Cellobiose by Enzymatic Hydrolysis.- Separation of Positional Isomers by Combined Use of Enzymatic Hydrolysis and Extraction.- Studies on the Synthesis and Properties of the Mimics with Glutathione Peroxidase Activity and Preparation of the Conjugate of Mimic-Antibody.- Direct Synthesis of Cyclodextrin in a Heterogeneous Enzyme Reaction System Containing Insoluble Extruded Starch.- 4. Fermentation Technology.- Engineering and Genetic Approaches to Optimization of Recombinant Fermentation Process.- Computer-Controlled Fed-Batch Cultivation of a Recombinant Escherichia coli for Overproduction of Human Interleukin-2.- Production of Recombinant HTLV-I Envelope Protein.- Computer-Controlled High Cell Density Cultivation of Recombinant Escherichia coli Producing ß-Galactosidase.- Use of an Adaptive Control Strategy for the Production of Exotoxin A from High Density Culture of Recombinant Escherichia coli.- Effective Extracellular Production of Heterologous Proteins by Automated Fed-Batch Cultures of Recombinant Microorganisms Carrying Excretion Plasmid Vectors.- Production of Antifungal Peptide Produced by Bacillus subtilis in Solid State Fermentation.- Dynamics of Trichoderma reesei Pellet Formation and Oxygen Penetration Phenomenon in the Pellet.- Studies on Drying Pullulan by Using Spray-Fluid-Bed with Carriers Circulation.- Molecular Breeding of a Biotin-Hyperproducing Strain of Serratia marcescens.- Hyper-Production of Thermostable Lipase by Genetically Engineered Pseudomonas Species.- Effects of In Vitro Protein Stabilizers on the Overproduction of ß-Lactamase in Escherichia coli.- Fermentation Conditions for Maximizing Hemolysin Production in Batch and Continuous Cultures of Recombinant Escherichia coli.- Reconsideration of Commercially Available Medium Component in Recombinant Yeast Culture.- Production of Hepatitis B Surface Antigen Including PreS(PreS-HBsAg) in Fed-Batch Culture of Recombinant Yeast.- Growth and Induction Effects on the Expression of rhTNF-ß.- Production of Thermophilic Protease (Aqualysin I) by Fed-Batch Cultures of Recombinant Escherichia coli.- Production of Poly-3-Hydroxybutyric Acid (PHB) by Alcaligenes eutrophus H16 in a Fed-Batch Culture.- Kinetic Analysis of Continuous and Batch Cultures with Streptococcus faecalis.- Kinetic Study of Lysine Fermentation in Cane Molasses Base Medium.- A New Scale-Up Method Based on the Effect of Ventilation on Aerated Fermentation Systems.- Measurement of Shaking-Flask Performance and Its Application to Scaling Up a Fermentation Process to a Stirred-Tank Fermentor.- Effect of Magnesium Sulfate on the Feedback Inhibition of Sisomicin Production.- Effect of pH and Its Optimization for High Molecular Weight of Pullulan.- Production of Biosurfactants by Candida Antarctica.- Photometric Measurement of High Cell Density Culture Broth with Continuous Dilution System.- Continuous and High Cell Density Cultivation of Escherichia coli W3110 (pEC901) Harboring Human Interferon Alpha A Gene.- Purification and Characterization of Nitrilase Catalyzing Asymmetric Hydrolysis of Aminonitriles from Rhodococcus rhodochrous PA-34.- Carbon Mass Balance Analysis of Sophorose Lipid Production by Torulopsis bombicola.- Gel Formation by Methylan Produced from Methanol.- 5. Plant Cell Culture.- Extractive Plant Cell Culture.- Studies on the Production of Secondary Metabolites in Plant Cell Culture.- Studies on Production of Flavonol Glycosides in Cell Cultures of Ginkgo biloba.- Flavonoid Production from a Cell Suspension Culture of Scutellaria baicalensis G.- Increasing Secondary Metabolite Production by In Vivo Extraction and Elicitation.- Investigation of the Production of Shikonin Derivatives from Immobilized Lithospermum erythrorhizon Cells by Intermittent Permeabilization.- Production of Red Pigments by Perilla frutescens Cells in Bioreactors.- Production of Plant Secondary Metabolites from High Density Perfusion Cultures.- Production of Artificial Seeds.- ß-Carotene Production by DO-Stat Semi-Continuous Culture of Carrot Cells in an Air-Lift Column with Draft Tube.- Effects of Bioregulators and Administration Methods on the Biotransformation of Monoterpenes by Cultured Cells of Eucalyptus perriniana.- Production of Cardenolides by Plant Cell and Tissue Cultures.- Effects of Rate Limiting Factors on Vitamin E Production Using Safflower Cells.- Stimulation of Shikonin Derivative Accumulation in Onosma paniculatum and Lithospermum erythrorhizon Cell Culture by Fungal Elicitors.- Production of Red Pigment by Tissue Culture of Phytolacca americana.- Production of Essential Oil by Mentha piperita Cell Culture.- Bioreactor-Based Culture of Plant Hairy Roots for Production and Recovery of Pigments.- Caffeine Production by Polyurethane Foam Immobilized Coffee (Coffea arabica L.) Cells.- Design of Tubular Microporous Membrane Aerated Bioreactors for Plant Cell Cultures.- 6. Animal Cell Culture.- Proteins and Pesticides from the Insect Cell-Baculovirus System.- Study of Hybridoma Cells, Kinetics in High Density Culture.- Physiological Modelling of Uptake and Partitioning of Glucose and Glutamine