Basic Confocal Microscopy

Basic Confocal Microscopy, Second Edition builds on the successful first edition by keeping the same format and reflecting relevant changes and recent developments in this still-burgeoning field. This format is based on the Confocal Microscopy Workshop that has been taught by several of the authors for nearly 20 years and remains a popular workshop for gaining basic skills in confocal microscopy. While much of the information concerning fluorescence and confocal microscopy that made the first edition a success has not changed in the six years since the book was first published, confocal imaging is an evolving field and recent advances in detector technology, operating software, tissue preparation and clearing, image analysis, and more have been updated to reflect this. Several of these advances are now considered routine in many laboratories, and others such as super resolution techniques built on confocal technology are becoming widely available.  

Chapter 1 Introduction and Historical Perspective.- Chapter 2 The Theory of Fluorescence.- Chapter 3 Fluorescence Microscopy.- Chapter 4 Specimen Preparation.- Chapter 5 Labeling Considerations for Confocal Microscopy.- Chapter 6 Digital Imaging.- Chapter 7 Confocal Digital Image Capture.- Chapter 8 Types of Confocal Instruments: Basic Principals and Advantages and Disadvantages.- Chapter 9 Setting the Operating Parameters.- Chapter 10 3D Reconstruction of Confocal Image Data.- Chapter 11 Analysis of Image Similarity and Relationship.- Chapter 12 Ethics and Resources.

Dr. W. Gray (Jay) Jerome is an Associate Professor of Pathology, Microbiology and Immunology at Vanderbilt University School of Medicine. He has published extensively on microscopy of biological material. His work involves correlating microscopic structural information with biochemical, physiological, and molecular genetic data to provide a comprehensive picture of structure-function relationships. Dr. Jerome is a Fellow of the Microscopy Society of America, a Fellow of the American Association for the Advancement of Science, a Fellow of the American Heart Association and a Past-President of the Microscopy Society of America.

Dr. Robert L. Price is a Research Professor in Cell Biology and Anatomy at the University of South Carolina School of Medicine and Director of the Instrumentation Resource Facility. He has been active in biomedical imaging using confocal microscopy since 1990 and along with Dr. Jerome has taught numerous Confocal Workshops in the United States and Australia.  Dr. Price has received several awards from the University of South Carolina and the Microscopy Society of America, is a Fellow of the Microscopy Society of America, and currently President of the Microscopy Society of America.