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This volume covers methods for determination of autoantibodies in rheumatic connective tissue diseases and organ-specific diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Autoantibodies: Methods and Protocols aims to be helpful for all persons working with research and development of autoimmune laboratory diagnostics and for clinicians using autoantibody tests in daily work with patients.1. Autoantibodies as Diagnostic Tools
Gunnar Houen
2. Non-specific Binding in Immunoassays for Autoantibodies
Gunnar Houen
3. Detection of Autoantibodies by Indirect Immunofluorescence Cytochemistry on HEp-2 Cells
Alessandra Dellavance and Luis Eduardo Coelho Andrade
4. Detection of Anti-Neutrophil Cytoplasmic Antibodies by Indirect Immunofluorescence
J. J. B. C. van Beers, J. Vanderlocht, C. Roozendaal, and Jan G. M. C. Damoiseaux
5. Determination of Subset-Restricted Anti-Neutrophil Cytoplasmic Antibodies (ANCA) by Immunofluorescence Cytochemistry
Firoozeh Amirbeagi, Amanda Welin, Pontus Thulin, and Johan Bylund
6. Determination of Anti-aquaporin 5 Autoantibodies by Immunofluorescence Cytochemistry
Jehan Alam, Sumin Jeon, and Youngnim Choi
7. Determination of cN1A Autoantibodies by Cell-Based Immunofluorescence Cytochemistry
Satoshita Yamashita and Nozomu Tawara
8. Determination of Agonistically Acting Autoantibodies to the Adrenergic Beta-1 Receptor by Cellular Bioassay
Hanna Davideit, Annekathrin Haberland, Sabine Bartel, Sarah Schulze-Rothe, Johannes Müller,and Katrin Wenzel
9. Determination of Autoantibodies to Salivary Gland Antigens
Li Cui, Xinyuan Zhao, and Shen Hu
10 Measurement of autoantibodies to the gastric H+,K+-ATPase (ATP4A/B) using a Luciferase Immuno Precipitation System (LIPS)
Edith Lahner, Ilaria Marzinotto, Cristina Brigatti, Howard Davidson, Janet Wenzlau, Lorenzo Piemonti, Bruno Annibale, and Vito Lampasona
11. Detection of DNA Autoantibodies by Electrophoretic Mobility Shift Assay
Jacqueline Keyhani and Ezzatollah Keyhani
12. Determination of Autoantibodies to Neutrophil Granule Constituents by Solid Phase Immunoassay
J. Vanderlocht, J.J.B.C. van Beers, P.C. Limburg, Jan G. M. C. Damoiseaux, and C. Roozendaal
13. Determination of Autoantibodies to C3 Nephritic Factors by ELISA
Lillemor Skattum
14. Determination of Anti-C1q Autoantibodies by Western Blot
Anci Verlemyr, Lennart truedsson, and Lillemor Skattum
15. Anti-Factor H Autoantibodies Assay by ELISA
Marie Sénant and Marie-Agnes Durey
16. Determination of Autoantibodies to Transglutaminase by Electrochemiluminescence (ECL) Assay
Zhiyuan Zhao, Yong Gu, Dongmei Miao, Eric Hoffmeyer, Yu Liu, and Liping Yu
17. Determination of CRP Autoantibodies by SPR Immunoassay
Li Qiu-yu and Li Hai-yun
18. Histone Protein Epitope Mapping for Autoantibody Recognition in Rheumatoid Arthritis
Felicia Real-Fernandez, Federico Pratesi, Paola Migliorini, and Paolo Rovero
19. Detection of SSA and SSB Autoantibodies by Enzyme-Linked Immunosorbent Assay
Nicole Hartwig Trier
20. Assessment of Peptidylarginine Deiminase Activity by ELISA using Human Fibrinogen as Substrate
Dres Damgaard and Claus H. Nielsen
21. The Use of a Citrullinated Peptide Panel for Detection of Anti-Citrullinated Protein Antibodies by Enzyme-Linked Immunosorbent Assay
Nicole H. Trier
22. Determination of Autoantibodies by Line Blotting
Louise Sternbæk, Tina Friis, and Gunnar Houen
23. Determination of Rheumatoid Factors by ELISA
Nicole H. Trier and Gunnar Houen
24. Detection of autoantibodies to complement components by surface plasmon resonance-based technology
Remi Noe, Sophie Chauvet, Shambhuprasad K. Togarsimalemath, Maria Chiara Marinozzi, Maria Radanova, Vasil V. Vasilev, Veronique Fremeaux-Bacchi, Marie-Agnes Dragon-Durey, and Lubka T. Roumenina
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