Advanced Techniques in Diagnostic Microbiology Volume 2: Applications

In recent years, advanced molecular techniques in diagnostic microbiology have been revolutionizing the practice of clinical microbiology in the hospital setting. Molecular diagnostic testing in general and nucleic acid-based amplification methods in particular have been heralded as diagnostic tools for the new millennium. This third edition covers not only the most recent updates and advances, but details newly invented omic techniques, such as next generation sequencing. It is divided into two distinct volumes, with Volume 1 describing the techniques, and Volume 2 addressing their applications in the field. In addition, both volumes focus more so on the clinical relevance of the test results generated by these techniques than previous editions. 

Bacterial identification based on universal gene amplification and sequencing.- Molecular techniques for blood and blood product screening.- Molecular diagnostics of sexual transmitted disease.- Advances in the diagnosis of Mycobacterium tuberculosis.- Advanced typing techniques in molecular epidemiology investigations.- Molecular detection and characterization of carbapenem-resistant Enterobacteriaceae.- Rapid Screening and Identification of MRSA.- Advanced methods for detection of food-borne pathogens.- Technical and clinical niches for point of care molecular devices.- Molecular diagnosis of emerging coronavirus infections.- Technical advances in veterinary diagnostic microbiology.- Recent advances in veterinary diagnostic virology.- Deep sequencing: technical advances and clinical microbiology applications.- Splicing RNA and application in clinical microbiology.- Application of microarrays for laboratory diagnosis of fungal infections.- Laboratory technical advances in diagnosis of Clostridium difficile infections.- Clostridium difficile infections in one health.- Technical advances in molecular diagnostics of HIV-1 infections.- Multiplex techniques for detection and identification of microbial pathogens.- Molecular diagnosis and monitoring of human papillomavirus infections.- Molecular niches for laboratory diagnosis of sepsis.- Advanced pathology techniques for emerging infectious disease pathogens.- Diagnosis and assessment of microbial infections with host miRNA profiles.- Microbiome in diagnosis and monitoring of microbial infections.- Whole genome sequencing for microbial pathogen detection and identificaiton.- Host immune repertoire and microbial infections.- Advanced techniques for antiviral drug resistance determination.- Test algorithms using advanced techniques.- Clinical interpretation and relevance of advanced technique results.

Yi-Wei Tang, Chief of Clinical Microbiology Service, Memorial Sloan-Kettering Cancer Center