Adult Stem Cells Methods and Protocols

This second edition volume expands on the previous edition with updated chapters covering a broader range of tissues and techniques pertaining to stem cell technologies. The chapters also cover topics such as the generation of iPSC-derived cells unique to the individual human genome addressing the possibility of more personalized clinical applications to an individual with a specific degenerative disease; and the use of nanoparticles such as 3D scaffolds and biomaterials as a means of improving stem cell viability after transplantation in the host tissue. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Adult Stem Cells: Methods and Protocols, Second Edition introduces insights into personalized medicine in stem cell therapies, and will spark new and innovative procedures relevant to stem cell therapy and tissue engineering.

Safe and Non-Invasive Method for Generating Induced Mesenchymal Stem Cells from Urinary Epithelial Cells.- A Non-Enzymatic Procedure to Obtain Human Mesenchymal Stromal Cells from the Dermis.- Adult Mesenchymal Stem Cells in Presence of Glycosaminoglycans.- Mitochondrial Transfer between Mesenchymal Stem Cells and Cancer Cells.- Isolation and Characterization of Human Dental Pulp Stem Cells Derived from Dental Pulp of Permanent Teeth.- A Three Step Protocol to Differentiate iPSC into Colon Organoids.- Comparison of Two Differentiation Protocols of Human Induced Pluripotent Stem Cells into Cardiomyocytes.- Enriching Cardiomyocytes Derived from hiPSCs by Magnetic-Activated Cell Sorting (MACS).- Generation of Induced Pluripotent Stem Cells from Erythoid Progenitor Cells.- iPSC-Based Disease Modelling and Functional Assessment of Neurons in Patients with Metabolic Disorder.- A Method for Producing Induced Pluripotent Stem Cell Derived Cardiomyocytes from Leigh Syndrome Patients for Its Application in Disease Modeling and Drug Validation.- Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Mononuclear Cells of C9ORF72-Associated Amyotrophic Lateral Sclerosis Patients.- Cardiac Progenitor Cell Metabolism.- Generation of Directly Reprogrammed Human Endothelial Cells.- Isolation and Characterization of Extracellular Vesicles from Cell Culture Media.- Exosomes Isolation, Purification, and Characterization.- Exosomes: Methods for Isolation and Characterization in Biological Samples.- Cancer Stem Cells: Detection and Characterization from Solid Tumors.- Generation of Bi-Dimensional and Three-Dimensional Muscle Culture Systems.- An Enzyme-Free Method for Isolation and Expansion of Muscle Stem Cells for Cultivated Meat Applications.- Synthesis of Hydrofluoric Acid (HF) Free, Two-Dimensional (2D) Tantalum Carbide MXene Nanosheets and Quantum Dots.- Three-Dimensional Electrically Conductive Scaffolds to Culture Cardiac Progenitor Cells.- Omic Evaluation of Nanomaterial Based Photodynamic Therapy of Cancer.- Electrospun Fibers for Use in Implantable Materials to Support Cell Therapy.- Caco-2 Monolayer as a Model of the Intestinal Barrier-Permeability of Magnesium Salts.- Antimicrobial Surface for Devices Used in Stem Culture Manipulation and In Vitro Biofabrication of Tissues.- Manufacturing Process of Prostheses using Semi-Rigid Moulds by Additive Technologies.- Preparation of Monodispersed Nanofibrous Gelatin Microspheres using Home-Built Microfluidics.