Adipose-Derived Stem Cells Methods and Protocols

During the past decade, a wide range of scientific disciplines have adopted the use of adipose-derived stem/stromal cells (ASCs) as an important tool for research and discovery. In Adipose-Derived Stem Cells: Methods and Protocols, experts from the field, including members of the esteemed International Federation of Adipose Therapeutics and Science (IFATS), provide defined and established protocols in order to further codify the utilization of these powerful and accessible cells. With chapters organized around approaches spanning the discovery, pre-clinical, and clinical processes, much of the emphasis is placed on human ASC, while additional techniques involving small and large animal species are included. As a volume in the highly successful Methods in Molecular Biology™ series, the detailed contributions include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Adipose-Derived Stem Cells: Methods and Protocols serves as a vital reference text for experienced researchers as well as new students on the path to further exploring the incredible potential of ASCs.

Part I: Isolation Methods 1. Isolation and Culture of Rhesus Adipose-Derived Stem Cells Christine Gagliardi and Bruce A. Bunnell 2. Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Yuan-Di C. Halvorsen, and Jeffrey M. Gimble 3. Isolation of Murine Adipose-Derived Stem Cells Gang Yu, Xiying Wu, Gail Kilroy, Yuan-Di C. Halvorsen, Jeffrey M Gimble, and Z. Elizabeth Floyd 4. In vitro Adult Rat Adipose Tissue-Derived Stromal Cell Isolation and Differentiation Mandi J. Lopez and Nakia D. Spencer 5. In vitro Adult Canine Adipose Tissue-Derived Stromal Cell Growth Characteristics Nakia D. Spencer and Mandi J. Lopez 6. Adipogenic Differentiation of Adult Equine Mesenchymal Stromal Cells Martin A. Vidal and Mandi J. Lopez 7. Isolation and Culture of Porcine Adipose Tissue-Derived Somatic Stem Cells Kellie J. Williams, Robert A. Godke, and Kenneth R. Bondioli 8. Automated Isolation and Processing of Adipose-Derived Stem and Regenerative Cells Kevin C. Hicok and Marc H. Hedrick Part II: Phenotyping Methods 9. Methods for the Purification and Characterization of Human Adipose-Derived Stem Cells Stan Gronthos and Andrew C.W. Zannettino 10. Characterization of Human ASCs Using Flow Cytometry H. Alan Tucker and Bruce A. Bunnell 11. Evaluation of Cellular and Humoral Immune Responses to Allogeneic Adipose-Derived Stem/Stromal Cells Kevin R. McIntosh 12. Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants Pauline Decaunes, David Estève, Alexia Zakaroff-Girard, Coralie Sengenès, Jean Galitzky, and Anne Bouloumié 13. Gel-Based and Gel-Free Proteomic Technologies Peter Scherp, Ginger Ku, Liana Coleman, and Indu Kheterpal Part III: Differentiation Methods: In vitro and In vivo Applications 14. Adipogenic Differentiation of Adipose-Derived Stem Cells Gang Yu, Z. Elizabeth Floyd, Xiying Wu, Teddi Hebert, Yuan Di C. Halvorsen, Ben Buehrer, and Jeffrey M. Gimble 15. 3D Culture Systems to Induce Chondrogenesis of Adipose-Derived Stem Cells Bradley T. Estes and Farshid Guilak 16. Neural Differentiation of Human ASCs Ji Min Yu, Bruce A. Bunnell, and Soo-Kyung Kang 17. Osteogenic Differentiation Strategies for Adipose-Derived Mesenchymal Stem Cells Robert Jan Kroeze, Marlene Knippenberg, and Marco N. Helder 18. Generation of Adipose Stromal Cell-Derived Hepatic Cells Joseph C. Ruiz 19. Adipose Stem Cell Differentiation into Smooth Muscle Cells Kacey G. Marra, Candace A. Brayfield, and J. Peter Rubin 20. Endothelial and Cardiac Regeneration from Adipose Tissues Louis Casteilla, Valérie Planat-Bénard, Stéphanie Dehez, Sandra De Barros, Corinne Barreau, and Mireille André 21. Epithelial Differentiation of Human Adipose-Derived Stem Cells Patrick C. Baer, Martin Brzoska, and Helmut Geiger 22. Screening for Epigenetic Target Genes that Enhance Reprogramming Using Lentiviral Delivered shRNA Jong S. Rim, Karen Strickler, Christian Barnes, Lettie Harkins, Jaroslaw Staszkiewicz, Rachel Power, and Kenneth J. Eilertsen Part IV: Bioengineering, Manufacturing, and Clinical Technologies 23. Adipogenic Differentiation of Human Adipose-Derived Stem Cells on 3D Silk Scaffolds Jennifer H. Choi, Evangelia Bellas, Gordana Vunjak-Novakovic, and David L. Kaplan 24. A First Approach for the Production of Human Adipose Tissue-Derived Stromal Cells for Therapeutic Use Philippe Bourin, Julie-Anne Peyrafitte, and Sandrine Fleury-Cappellesso 25. Viral Transduction of Adipose-Derived Stem Cells Patricia A. Zuk 26. Use of Adipose-Derived Stem Cells in High Throughput Screening to Identify Modulators of Lipogenesis Y. Reneé Lea-Currie, David J. Duffin, and Benjamin M. Buehrer 27. Preservation Protocols For Human Adipose Tissue-Derived Adult Stem Cells Ram Devireddy and Sreedhar Thirumala 28. Soft Tissue Reconstruction J. Peter Rubin and Kacey G. Marra 29. Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Adipose Tissue-Derived Mesenchymal Stem Cells Yeon Jeong Kim and Jin Sup Jung 30. The Generation and the Manipulation of Human Multipotent Adipose-Derived Stem Cells Brigitte Wdziekonski, Tala Mohsen-Kanson, Phi Villageois, and Christian Dani 31. Cell Sheet Technology for Tissue Engineering: The Self-Assembly Approach Using Adipose-Derived Stromal Cells Benoît Labbé, Guillaume Marceau-Fortier, and Julie Fradette 32. Differentiation of Adipose-Derived Stem Cells for Tendon Repair A. Cagri Uysal and Hiroshi Mizuno 33. Adipose-Derived Stem Cells for Skin Regeneration Hiroshi Mizuno and Masaki Nambu 34. Adipose-Derived Stem Cells for Periodontal Tissue Regeneration Morikuni Tobita and Hiroshi Mizuno