Adipose-Derived Stem Cells Methods and Protocols

This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Adipose-Derived Stem Cells: Methods and Protocols, Second Edition is a valuable resource for anyone interested in learning more about the scientific advances in the field of ASC biology and application.

Preface…

Table of Contents…

Contributing Authors…

1. Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue

Annie C. Bowles, Alan Tucker, and Bruce A. Bunnell

2. A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Timeframe

Joel A. Aronowitz, Ryan A. Lockhart, and Cloe S. Hakakian

3. Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells

4. The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells

Anne-Laure Hafner, Tala Mohsen-Kanson, and Christian Dani

5. Methylcellulose Based Thermally Reversible Hydrogels

Anoosha Forghani and Ram Devireddy

6. Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery 

Pascal Morissette Martin, Arthi Shridhar, Claire Yu, Cody Brown, and Lauren E. Flynn

7. Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells 

Anthony D. Foster, Nicholas Clark, and Thomas A. Davis

8. High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes

Dominique Mayrand and Julie Fradette

 

9. Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery 

Pietro Gentile and Valerio Cervelli 

10. Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction 

Pinar Yilgor Huri, Jordana Gilbert-Honick, Justin Morrissette-McAlmon, Sarah 

Somers, and Warren L. Grayson

11. Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis

Gail Kilroy, Marilyn Dietrich, Xiying Wu, Jeffrey M. Gimble, and Z. Elizabeth Floyd

12. Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue 

Hubert Tseng, Alexes C. Daquinag, Glauco R. Souza, and Mikhail G. Kolonin

13. Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates

Jie Li, J. Lowry Curley, Z. Elizabeth Floyd, Xiying Wu, Yuan Di C. Halvorsen, and Jeffrey M. Gimble

14. Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method 

Jie Li, Hui Li, and Weidong Tian

15. Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation 

Carmel Fargason, Nan Zhang, and Mandi J. Lopez

16. Soft Tissue Reconstruction

Kacey G. Marra

17. Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain 

Rachel C. Nordberg, Josie C. Bodle, and Elizabeth G. Loboa

18. Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells 

Shahensha Shaik and Ram Devireddy 

19. Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma   

Satoshi Tajima, Morikuni Tobita, and Hiroshi Mizuno

20. Isolation and Differentiation of Murine Primary Brown/Beige Preadipocytes  

Hui Yu, Margo Emont, Heejin Jun, and Jun Wu