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oliver guillermo (curatore); kahn mark l. (curatore) - lymphangiogenesis
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Lymphangiogenesis Methods and Protocols

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Dettagli

Genere:Libro
Lingua: Inglese
Editore:

Humana

Pubblicazione: 09/2018
Edizione: 1st ed. 2018





Trama

This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field.






Sommario

Preface…
Table of Contents…
Contributing Authors…

1. Three-Dimensional Visualization of the Lymphatic Vasculature
Cathrin Dierkes, Aaron Scherzinger, and Friedermann Kiefer

2. The Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels
Kelly L. Betterman and Natasha L. Harvey

3. Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice
Ines Martinez-Corral and Taija Makinen

4. Visualization and Tools for Analysis of Zebrafish Lymphatic Development Kazuhide S. Okuda, Sungmin Baek, and Benjamin M. Hogan

5. Characterization of Zebrafish Facial Lymphatics
Tiffany C.Y. Eng and Jonathan W. Astin

6. Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development
Xin Geng and R. Sathish Srinivasan

7. Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function Amélie Sabine, Michael J. Davis, Esther Bovay, and Tatiana V. Petrova

8. Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice Sang Heon Suh, Seon Pyo Hong, Intae Park, Joo-Hye Song, and Gou Young Koh

9. Morphological and Functional Analysis of CNS Associated Lymphatics
Jasmin Herz, Antoine Louveau, Sandro Da Mesquita, and Jonathan Kipnis

10. Morphological Analysis of Schlemm's Canal in Mice
Benjamin R. Thomson and Susan E. Quaggin

11. Imaging Lymphatics in Mouse Lungs
Peter Baluk and Donald M. McDonald

12. Imaging of Endothelial Cell Dynamic Behavior in Zebrafish
Baptiste Coxam and Holger Gerhardt

13. Visualization and Measurement of Lymphatic Function In Vivo
Samia B. Bachmann, Michael Detmar, and Steven T. Proulx

14. Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells
Daniel T. Sweet, Joshua D. Hall, John Welsh, Mark L. Kahn, and Juan M. Jiménez

15. Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo
Jorge A. Castorena-Gonzalez, Joshua P. Scallan, and Michael Davis

16. Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior
Jocelynda Salvador and George E. Davis

17. Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis
Witold W. Kilarski, Esra Güç, and Melody A. Swartz

18. Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro
Anita Rogic, Francois Auger, and Mihaela Skobe

19. Stimulation and Inhibition of Lymphangiogenesis via Adeno-Associated Viral Gene Delivery
Sinem Karaman, Harri Nurmi, Salli Antila, and Kari Alitalo

20. Isolation and Characterization of Mouse Organ Specific Endothelial Transcriptomes
Chaitanya Badwe, Shahin Rafii, and Brisa Palikuqi

21. Single Cell mRNA Sequencing of the Mouse Brain Vasculature
Michael Vanlandewijck and Christer Betsholtz

22. Metabolic Analysis of Lymphatic Endothelial Cells
Pengchun Yu, Tiago C. Alves, Richard G. Kibbey, and Michael Simons

23. Characterizing Epigenetic Changes in Endothelial Cells
Matthew T. Menendez and Courtney T. Griffin














Altre Informazioni

ISBN:

9781493987115

Condizione: Nuovo
Collana: Methods in Molecular Biology
Dimensioni: 254 x 178 mm Ø 900 gr
Formato: Copertina rigida
Illustration Notes:XII, 345 p. 93 illus., 81 illus. in color. With online files/update.
Pagine Arabe: 345
Pagine Romane: xii


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