Type-1 Diabetes

TRAMA

This volumes details methods focusing on technological innovation and recent advances in diabetes management. Chapters will guide readers through recent advances, beta-cell regeneration, non-invasive imaging of endogenous, transplanted islets theranostics, microRNA profiling of beta-cells and artificial intelligence, and deep learning algorithms in diabetes. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Type-1 Diabetes: Methods and Protocols is for a broad audience including basic researches, clinicians, and physician scientists whose major focus is in diabetes.

SOMMARIO

1. 3D optical molecular imaging of the rodent pancreas by OPT and LSFM Max Hahn and Ulf Ahlgren   3. Uncommon Transplantation Sites – Transplantation of islets and islet organoids in the anterior chamber of the eye of rodents and monkeys Tilo Moede, Sai Bo Bo Tun, Ingo B. Leibiger, and Per-Olof Berggren   3. Generation of Insulin-producing multicellular organoids Laura Mar-Fonseca, Fanny Lebreton, Charles-Henri Wassmer, and Ekaterine Berishvili   4. Non-invasive quantitative PET Imaging in humans of the Pancreatic Beta Cell Mass biomarkers VMAT2 and Dopamine, D2/D3 receptors, in vivo. Jason Bini, Richard E Carson, and Gary W Cline   5. In Vivo Imaging of Naked and Microencapsulated Islet Cell Transplantation Dian R. Arifin and Jeff W.M. Bulte   6. Diabetes retinopathy: new ways to detect and treat Todd A. Lydic and Julia V. Busik   7. MRI methods for imaging beta-cell function in the rodent pancreas Pooyan Khalighinejad, Eul Hyun Suh, and A. Dean Sherry   8. MicroRNAs in type 2 diabetes – focus on microRNA profiling in Islets of Langerhans Elaine Cowan, Alexandros Karagiannopoulos, Lena Eliasson   9. Protocol for clinical GLP-1 receptor PET/CT imaging with [68Ga]Ga-NODAGA-exendin-4 S. Tokgöz, M. Boss, S. Prasad, P. Shah, P. Laverman, M. van Riel, and M. Gotthardt   10. Hyperinsulinemic-euglycemic clamp in conscious rats based on the tail artery and vein catheterization Xiaohuan Liu, LuLu Chen, and Xiang Hu   11. Synthesis of siRNA-conjugated dextran-coated iron oxide nanoparticles for islet protection during transplantation and noninvasive imaging. Neil Robertson, Ping Wang, Nazanin Talebloo Kazuhiko Yamada, and Anna Moore   12. Micelle-Based Nanocarriers for Targeted Delivery of Cargo to Pancreas Swati Mishra and Philip R. Streeter 13. Magnetic particle imaging of transplanted human islets using a machine learning algorithm Aixia Sun, Hasaan Hayat, Simon W. Sanchez, Anna Moore, and Ping Wang    14. In vivo bioluminescence for the detection of the fate of pancreatic islet organoids post-transplantation Aixia Sun, Elizabeth Kenyon, Mithil Gudi, Wen Li, Aitor Aguirre, and Ping Wang   15. miRNA theranostic nanoparticles promote pancreatic beta cell proliferation in type 1 diabetes model Saumya Nigam, Anna Moore, and Ping Wang   16. Isolation and purification of human pancreatic islets LingLing Wei   17. Vascularized islet transplantation as composite Islet-Kidney grafts with nanoparticle-labeled islets in large animal preclinical transplant models Kazuhiko Yamada, Daniel L. Eisenson, Xiaojuan Chen, Lei Ji, Michelle R. Santillan, and Anna Moore    

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071628065
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 700 gr
• Formato: Copertina rigida
• Illustration Notes: XII, 252 p. 54 illus., 49 illus. in color.
• Pagine Arabe: 252
• Pagine Romane: xii