Imaging Flow Cytometry

TRAMA

This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.  Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

SOMMARIO

Part I: General 1. Quantitative Functional Morphology by Imaging Flow Cytometry            Ivan A. Vorobjev and Natasha S. Barteneva 2. Principles of Amnis Imaging Flow Cytometry            David A. Basiji 3. Ultrafast Microfluidic Cellular Imaging by Optical Time-Stretch            Andy K.S. Lau, Terence T.W. Wong, Ho Cheung Shum, Kenneth K.Y. Wong, and Kevin K. Tsia 4. Applications of Imaging Flow Cytometry for Microalgae            Mark Hildebrand, Aubrey Davis, Raffaela Abbriano, Haley R. Pugsley, Jesse C. Traller, Sarah R. Smith, Roshan P. Shrestha, Orna Cook, Eva Sanchez, Kalpana Manandhar-Shrestha, and Benjamin Alderete Part II: IFC in Detection of Nuclei and Nucleic Acids 5. The Analysis of Cell Cycle, Proliferation, and Asymmetric Cell Division by Imaging Flow Cytometry            Andrew Filby, William Day, Sukhveer Purewal, and Nuria Martinez-Martin 6. Quantitation of Chromosome Damage by Imaging Flow Cytometry            Lindsay A. Beaton-Green and Ruth C. Wilkins 7. Fluorescent In Situ Hybridization In Suspension by Imaging Flow Cytometry            Orla Maguire, Paul K. Wallace, and Hans Minderman 8. Analysis of Nucleo-Cytoplasmic Protein Shuttling by Imaging Flow Cytometry            Elizaveta Fasler-Kan, Yeldar Baiken, Ivan Vorobjev, and Natasha S. Barteneva 9. Using Image-Based Flow Cytometry with a FISH-Based FlowRNA Assay to Simultaneously Detect Intracellular TNF-a Protein and mRNA in Monocytes Following LPS Stimulation            Adam S. Venable, Andrea L. Henning, Eric A. Prado, and Brian K. McFarlin Part III: IFC in Immune Response and Drug Screening 10. Multiparametric Characterization of Human T-Cell Immune Synapses by InFlow Microscopy            Guido H. Wabnitz and Yvonne Samstag 11. Studying T Cells N-Glycosylation by Imaging Flow Cytometry            Ana M. Dias, Catarina R. Almeida, Celso A. Reis, and Salomé S. Pinho 12. Assessment of Granulocyte Subset Activation: New Information from Image-Based Flow Cytometry            Brian K. McFarlin, Eric A. Prado, Adam S. Venable, Andrea L. Henning, and Randall R. Williams 13. Using Image-Based Flow Cytometry to Assess Monocyte Oxidized LDL Phagocytosis Capacity            Andrea L. Henning, Adam S. Venable, Eric A. Prado, and Brian K. McFarlin 14. Screening for Drugs Against the Plasmodium falciparum Digestive Vacuole by Imaging Flow Cytometry            Yan Quan Lee, Brian E.Hall, and Kevin S.W. Tan Part IV: IFC in Studies of Cell Death and Apoptosis 15. Accurate Assessment of Cell Death by Imaging Flow Cytometry            Aja M. Rieger and Daniel R. Barreda 16. Temporal Heterogeneity in Apoptosis Determined by Imaging Flow Cytometry            Ivan A. Vorobjev and Natasha S. Barteneva Part V: Imaging Cytometry in Cell Classification and Morphological Analysis 17. FlowCAM: Quantification and Classification of Phytoplankton by Imaging Flow Cytometry            Nicole J. Poulton 18. Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry            Leigh Samsel and J. Philip McCoy, Jr. 19. Imaging Flow Cytometric Analysis of Primary Bone Marrow Megakaryocytes            Lisa M. Niswander, James Palis, and Kathleen E. McGrath 20. Sickle Cell Imaging Flow Cytometry Assay (SIFCA)            Kleber Y. Fertrin, Leigh Samsel, Eduard J. van Beers, Laurel Mendelsohn, Gregory J. Kato, and J. Philip McCoy

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493949014
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 5888 gr
• Formato: Brossura
• Illustration Notes: XIII, 295 p. 89 illus., 86 illus. in color.
• Pagine Arabe: 295
• Pagine Romane: xiii