Cereal Genomics

TRAMA

In Cereal Genomics: Methods and Protocols, expert researchers provides modern protocols for the analysis and manipulation of cereal genomes.  Techniques for isolation and analysis of DNA and RNA from both the vegetative tissues and from the more challenging seeds of cereals are described.  Tools for the isolation, characterization and functional analysis of cereal genes and their transcripts are detailed. Methods for molecular screening of cereals and for their genetic transformation are also covered. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Cereal Genomics: Methods and Protocols provides a comprehensive resource for those studying cereal genomes.

SOMMARIO

 1. Dna Extraction from Vegetative Tissue for Next Generation SequencingAgnelo Furtado2. DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples) Chiaki Mutou, Katsunori Tanaka, Ryuji Ishikawa 3. RNA Extraction from Cereal Vegetative TissueJulie A. Pattemore 4. RNA Extraction from Developing or Mature Wheat SeedsAgnelo Furtado 5. cDNA Library PreparationMaarten Kooiker and Gang-Ping Xue 6. Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in PlantsSiddanagouda S. Biradar, Xiaojun Nie, Kewei Feng and Song Weining 7. The Polymerase Chain Reaction (PCR) - General MethodsDaniel L. E. Waters and Frances M. Shapter 8. Mutation and Mutation ScreeningL. Slade Lee, Bradley J. Till,  Helen Hill, Owen A. Huynh, and Joanna Jankowicz-Cieslak 9. The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene ExpressionTimothy L. Fitzgerald and Richard B. McQualter 10. Cloning of DNA Fragments: Ligation Reactions in Agarose Gel  Agnelo Furtado11. Rapid Cloning of Genes and Promoters for Functional AnalysesPeer M. Schenk 12. Genome WalkingFrances M. Shapter and Daniel L.E. Waters 13. Functional Analysis by Protein BiochemistryLouis M. T. Bradbury 14. Genomic Southern Blot AnalysisLeigh Gebbie 15. Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs Shazia Iram16. Protein Blotting-Protocol for BeginnersLars A. Petrasovits 17. Genetic Transformation of Wheat via Particle BombardmentCaroline A. Sparks & Huw D. Jones 18. Sorghum Genetic Transformation by Particle Bombardment Guoquan Liu, Bradley C. Campbell and Ian D. Godwin 19. Genetic Transformation of Wheat via Agrobacterium-mediated DNA DeliveryCaroline A. Sparks, Angela Doherty & Huw D. Jones 20. A Protocol for High-throughput Agrobacterium-mediated Barley Transformation Wendy A Harwood 21. Agrobacterium-mediated Transformation- Rice TransformationInez H. Slamet-Loedin, Prabhjit Chadha-Mohanty, Lina Torrizo  22. Agrobacterium-mediated Transformation of Maize (Zea mays) Immature EmbryosHyeyoung Lee and Zhanyuan J. Zhang 23. A Technical Platform for PCR-based SNP Screening in Cereals and Other CropsZining Wang 24. A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing DataGopala Krishnan, S., Daniel L. E. Waters and Robert J. Henry 

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781627037143
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 7318 gr
• Formato: Copertina rigida
• Illustration Notes: X, 300 p. 39 illus., 29 illus. in color.
• Pagine Arabe: 300
• Pagine Romane: x