Cell Cycle Oscillators

TRAMA

This volume brings together a unique collection of protocols that cover standard, novel, and specialized techniques. Cell Cycle Oscillators: Methods and Protocols guides readers through recent progress in the field from both holistic and reductionist perspectives, providing the latest developments in molecular biology techniques, biochemistry, and computational analysis used for studying oscillatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Cell Cycle Oscillators: Methods and Protocols will serve as an invaluable reference to gain further insight into the complex and incompletely understood processes that are involved in the cell cycle and its regulation by oscillatory networks.

SOMMARIO

Part I Reviews1. Cell Cycle Control: A System of Interlinking OscillatorsRandy Y.C. Poon 2. Model Organisms for Studying the Cell CycleZhaohua Tang 3. Role of Computational Modelling in Understanding Cell Cycle OscillatorsAttila Csikász-Nagy and Ivan Mura 4. E2F Transcription Factors Control the Roller Coaster Ride of Cell Cycle Gene ExpressionIngrid Thurlings and Alain de Bruin Part II Methods 5. Cell Synchronization of Mouse Embryonic FibroblastsMichael J. Thwaites, Courtney H. Coschi, Christian E. Isaac, and Frederick A. Dick 6. Cell Cycle Synchronisation in Xenopus Egg Extracts Peter J. Gillespie, Julia Neusiedler, Kevin Creavin, Gaganmeet Singh Chadha,  and J. Julian Blow 7. Elutriation for Cell Cycle Synchronization in Fission YeastKazunori Kume 8. Spatiotemporal Investigation of Phosphorylation Events during Cell Cycle ProgressionLilia Gheghiani and Olivier Gavet 9. Cell Cycle Dynamics of Proteins and Post-translational Modifications Using Quantitative ImmunofluorescenceKaren Akopyan, Arne Lindqvist, and Erik Müllers 10. Building a Synthetic Transcriptional OscillatorMatthaeus Schwarz-Schilling, Jongmin Kim, Christian Cuba, Maximilian Weitz, Elisa Franco, Friedrich C. Simmel 11. Time Series Simulations and Bifurcation Analyses of a Model of Mitotic Oscillations in Xenopus Cell-free ExtractsTongli Zhang  12. A Computational Method for Identifying Yeast Cell Cycle Transcription FactorsWei-Sheng Wu 13. Measuring Activity and Specificity of Protein PhosphatasesBrendan L. Powers, Michael Melesse, Christie L. Eissler, Harry Charbonneau, and Mark C. Hall 14. Combining the Optimized Yeast Cytosine Deaminase Protein Fragment Complementation Assay and an in vitro Cdk1 Targeting Assay to Study the Regulation of the g-Tubulin ComplexPo Hien Ear, Jacqueline Kowarzyk , Michael J. Booth, Diala Abd-Rabbo, Kristian Shulist, Conrad Hall, Jackie Vogel, and Stephen W. Michnick 15. Cell Cycle Synchronisation Using a Microfluidic Synchroniser for Fission Yeast CellsShujing Wang and Chunxiong Luo 16. Detection of Protein-Protein Interactions in Tobacco BY-2 cells Using BimolecularFluorescence Complementation Gemma S. Puts and Natasha Spadafora 17. Tracking the Cyclin B1-GFP Sensor to Profile the Pattern of Mitosis Versus Mitotic BypassVictoria Griesdoorn, M Rowan Brown, Marie Wiltshire, Paul J Smith, and Rachel J Errington 18. Measuring APC/C Dependent Ubiquitylation in vitroMarc A. Jarvis, Nicholas G. Brown, Edmond R. Watson, Ryan VanderLinden, Brenda A. Schulman, and Jan-Michael Peters 19. Using the Fly-FUCCI System for the Live Analysis of Cell Cycle Dynamics in Cultured Drosophila Cells Zielke, N., van Straaten, M. and Edgar, BA 20. Imaging Cell Cycle Phases and Transitions of Living Cells From Yeast to WomanHadas Segev, Drora Zenvirth, Kobi J. Simpson-Lavy, Naomi Melamed-Book,  and Michael Brandeis 21. Measurement of Cdk1/cyclin B Kinase Activity by Specific Antibodies and Western BlottingCody W. Lewis, Ryan G. Taylor, and Roy M. Golsteyn 22. The use of SNAP Labeling to Study Cell Cycle Oscillatory ProteinsChristine Greil, Marie Follo, Monika Engelhardt, and Ralph Wäsch 

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493929566
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 9171 gr
• Formato: Copertina rigida
• Illustration Notes: XII, 352 p. 80 illus., 60 illus. in color.
• Pagine Arabe: 352
• Pagine Romane: xii