Synthetic Biology

TRAMA

 In Synthetic Biology, expert researchers in the field provide the latest developments in molecular biology techniques used in Synthetic Biology.  Focusing on computational tools that will aid in systematising the design and construction of parts and systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Synthetic Biology seeks to aid scientists in the further study of developing new biological components and systems.

SOMMARIO

Part I: Introduction1. What is Synthetic Biology?Karen M Polizzi Part II: DNA Assembly2. Gene Synthesis Method Based on Overlap Extension PCR and DNAWorks ProgramGang Li, Bing-Xue Dong, Yu-Huan Liu, Chang-Jie Li, and Li-Ping Zhang 3. BioBrick™ Assembly Using the In-Fusion PCR Cloning KitSean C. Sleight and Herbert M. Sauro 4. Overlap Extension PCR CloningAnton Bryksin and Ichiro Matsumura 5. One-Step Isothermal Assembly of DNA FragmentsRui TL Rodrigues and Travis S Bayer  PartIII: Part, Pathway, and Genome Engineering6. Creation and Characterization of Component Libraries for Synthetic BiologyTim Weenink and Tom Ellis 7. In vivo and in vitro Characterisation of s70 Constitutive Promoter BioParts by Real-time PCR and Fluorescent MeasurementsJames Chappell and Paul Freemont 8. In Vivo Screening of Artificial Small RNAs for Silencing Endogenous Genes in Escherichia coliVandana Sharma and Yohei Yokobayashi 9. Construction and Engineering of  Large Biochemical Pathways via DNA AssemblerZengyi Shao and Huimin Zhao10. Assembly of Multi-gene Pathways and Combinatorial Pathway Libraries through ePathBrick VectorsPeng Xu and Mattheos A.G. Koffas 11. Tandem Recombineering by SLIC Cloning and Cre-LoxP Fusion to Generate Multigene Expression Constructs for Protein Complex Research Matthias Haffke, Cristina Viola, Yan Nie, and Imre Berger 12. Combinatorial DNA Assembly using Golden Gate CloningCarola Engler and Sylvestre Marillonnet 13. Construction of Synthetic Gene Circuits in the Escherichia coli GenomeBei-Wen Ying, Yuya Akeno, and Tetsuya Yomo 14. Shuffling of DNA Cassettes in a Synthetic IntegrinDavid Bikard and Didier Mazel  Part IV: Computational Tools for Modelling Biological Systems15. Systematic Methodology for the Development of Mathematical Models for Biological ProcessesCleo Kontoravdi  16. SOBOLHDMR: A General Purpose Modelling SoftwareDr Sergei Kucherenko

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493962860
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 4511 gr
• Formato: Brossura
• Illustration Notes: X, 226 p. 80 illus., 48 illus. in color.
• Pagine Arabe: 226
• Pagine Romane: x