Spectral and Imaging Cytometry

TRAMA

This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations.Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

SOMMARIO

Dedication…Preface…Table of Contents…Contributing Authors…Part I Spectral Flow Cytometry1. Development of Spectral Imaging CytometryIvan A. Vorobjev, Aigul Kussanova, and Natasha S. Barteneva2. Using Virtual Filtering Approach to Discriminate Microalgae by Spectral Flow CytometerNatasha S. Barteneva, Aigul Kussanova, Veronika Dashkova, Ayagoz Meirkhanova, and Ivan A. VorobjevPart II Imaging Flow Cytometry - Imagestream3. Imaging Flow Cytometric Analysis of Primary Bone Marrow Erythroblastic IslandsJoshua Tay, Kavita Bisht, Ingrid G. Winkler, Jean-Pierre Levesque4. Imaging Flow Cytometry of Legionella-Containing Vacuoles in Intact and Homogenized Wild-Type and Mutant DictyosteliumAmanda Welin, Dario Hüsler, and Hubert Hilbi5. Imaging Flow Cytometry of Multi-NuclearityIvan A. Vorobjev, Sultan Bekbayev, Adil Temirgaliyev, Madina Tlegenova, and Natasha S. Barteneva6. The Imaging Flow Cytometry-Based Cytokinesis-Block MicroNucleus (CBMN) Assay  Ruth C. Wilkins, Matthew Rodrigues, and Lindsay A. Beaton-Green7. High-Throughput ?-H2AX Assay Using Imaging Flow CytometryYounghyun Lee, Qi Wang, Ki Moon Seong, and Helen C. Turner8. Label Free Identification of Persistent Particles in Association with Primary Immune Cells by Imaging Flow CytometryBradley Vis, Jonathan J. Powell, and Rachel E. Hewitt9. “Immuno-FlowFISH": Applications for Chronic Lymphocytic LeukaemiaHenry Y.L. Hui, Wendy N. Erber, and Kathy A. Fuller10. Quantifying Golgi Apparatus Fragmentation Using Imaging Flow Cytometry Inbal Wortzel and Ziv Porat11. Flow Imaging of the Inflammasome: Evaluating ASC Speck Characteristics and Caspase-1 Activity Abhinit Nagar and Jonathan A. Harton12. Quantitative Analysis of Latex Beads Phagocytosis by Human Macrophages Using Imaging Flow Cytometry with Extended Depth of Field (EDF) Ekaterina Pavlova, Daria Shaposhnikova, Svetlana Petrichuk, Tatiana Radygina, and Maria ErokhinaPart III Imaging Flow Cytometry - FlowCam13. FlowCam 8400 and FlowCam Cyano Phytoplankton Classification and Viability Staining by Imaging Flow CytometryKathryn H. Roache-Johnson and Nicole R. Stephens14. Optimizing FlowCam Imaging Flow Cytometry Operation for Classification and Quantification of Microcystis MorphospeciesDmitry Malashenkov, Veronika Dashkova, Ivan A. Vorobjev, and Natasha S. BartenevaSubject Index List…

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071630198
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 722 gr
• Formato: Copertina rigida
• Illustration Notes: XIV, 260 p. 1 illus.
• Pagine Arabe: 260
• Pagine Romane: xiv