Regulatory T-Cells

TRAMA

This volume provides methods and models for investigating the immunosuppressive subset of CD4+ T-cells, regulatory T-cells (Treg). Chapters guide readers through, basic protocols for analyzing Treg in mice and humans, single-cell analysis for analyzing Treg, and methods for the analysis of Treg in clinical application. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Regulatory T-Cells: Methods and Protocols aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge. 

SOMMARIO

Part I     Immunological techniques for analysing Treg 1.       Analysis of the in vivo function of follicular regulatory T (TFR) cells in the regulation of antibody response Lin Luo and Jianmei W. Leavenworth 2.       Analysis of peripherally derived Treg in the intestines Tadashi Takeuchi and Hiroshi Ohno 3.       Isolation and analysis of tumor infiltrating Treg Katharina Bergerhoff 4.       Induction of peripheral Treg José Almeida-Santos, Marie-Louise Bergman, and Jocelyne Demengeot 5.       Adoptive transfer and bone marrow chimera models to analyse Treg function and differentiation       Vital da Silva Domingues, Iris Caramalho, Marie-Louise Bergman, and Jocelyne Demengeot   Part III         Molecular techniques for analysing Treg 6.       Functional analysis of Foxp3 and its mutants by retroviral transduction of murine primary CD4+ T cells  Akira Nakajima, Ryuichi Murakami, Shohei Hori 7.       Genetic Tools for Analyzing Foxp3+ Treg cells: Fluorochrome-Based Transcriptional Reporters and Genetic Fate-Mapping Mario Simonetti, Acelya Yilmazer, and Karsten Kretschmer 8.       Depletion of Treg by the Diphtheria Toxin System Sho Hanakawa, Zachary Chow, and Kenji Kabashima 9.       Analysis of FOXP3 DNA methylation patterns to identify functional FOXP3+ T-Cell subpopulations Ekaterina Minskaia and João F. Lacerda 10.   Protein Ubiquitination Assay Ping Wei, Rui Liang, and Fan Pan   Part IV         Human Treg analysis and clinical application 11.   Phenotypic and Functional Studies of Human Treg cell Subpopulations Mo Atif, Mustapha Cherai, Makoto Miyara 12.   In vitro analysis of CTLA-4 mediated transendocytosis by Regulatory T cells Erin Waters, Cayman Williams, Alan Kennedy, and David M Sansom 13.   Isolation and functional characterisation of regulatory CD4+ T cells from the inflamed joints of patients with rheumatoid arthritis Giovanni A.M. Povoleri, Veerle Fleskens and Leonie S. Taams 14.   A GMP protocol for the manufacture of regulatory T cells for clinical application Alice O’Neil, Matthew Brook, Seetha Abdul-Wahab, Joanna Hester, Giovanna Lombardi and Fadi Issa   Part V Single cell and high-dimensional techniques for analysing Treg 15.   Regulatory T-cell phenotyping using CyTOF Hugo Barcenilla, Mikael Pihl, Florence Sjögren, Louise Magnusson, and Rosaura Casas 16.   High-dimensional single-cell profiling of tumor-infiltrating CD4+ regulatory T cells Giorgia Alvisi, Simone Puccio, Rahul Roychoudhuri, Caterina Scirgolea and Enrico Lugli 17.   Single-cell transcriptome analysis of Treg Benjy Tan Jek Yang, Masahiro Ono, and Yorifumi Satou

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781071626467
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 751 gr
• Formato: Copertina rigida
• Illustration Notes: XI, 280 p. 52 illus., 42 illus. in color.
• Pagine Arabe: 280
• Pagine Romane: xi