MicroRNA and Cancer

TRAMA

This volume details basic principles of experimental and computational methods for the study of microRNAs in cancer research and, therefore, provides a firm grounding for those who wish to develop further applications. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, MicroRNA and Cancer: Methods and Protocols, Second Edition aims to ensure successful results in the further study of this vital field

SOMMARIO

Part I: Review 1. Non- coding RNAs in DNA Damage Response: Opportunities for Cancer Therapeutics Wani Arjumand, Asia Asiaf, and Shiekh Tanveer Ahmad   2. MicroRNAs in Breast Cancer: Diagnostic and Therapeutic Potential Asia Asiaf, Shiekh Tanveer Ahmad, Wani Arjumand, and Mohammad Afzal Zargar   3. Involvement of miRNAs and Pseudogenes in Cancer Lütfi TUTAR, Aykut ÖZGÜR, and Yusuf TUTAR   4. microRNAs Reprogram Tumor Immune Response Wenfang Cheng and Wei Wu   5. APOBEC Gene Expression Regulated by microRNAs Wei Cao and Wei Wu   6. MicroRNAs Changes the Landscape of Cancer Resistance Jun Zhu and Wei Wu   7. MicroRNA, Noise, and Gene Expression Regulation Wei Wu     PART II: Protocol Section 8. Deep Sequencing Reveals a microRNA Expression Signature in Triple-negative Breast Cancer Yao-Yin Chang, Liang-Chuan Lai, Mong-Hsun Tsai, and Eric Y. Chuang   9. Detection of Plasma microRNA Signature in Osteosarcoma Patients Wendy Allen-Rhoades and Jason T. Yustein   10. Identification of E6/E7-dependent microRNAs in HPV-positive Cancer Cells Anja Honegger, Daniela Schilling, Holger Sültmann, Karin Hoppe-Seyler, and Felix Hoppe-Seyler   11. Combination of Anti-miRNAs Oligonucleotides with Low Amounts of Chemotherapeutic Agents for Pancreatic Cancer Therapy Marta Passadouro and Henrique Faneca   12. Evaluation of microRNA Delivery in vivo Rikki A. M. Brown, Kirsty L. Richardson, Felicity C. Kalinowski, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Marisa H. De Pinho, Dianne J. Beveridge, Lisa M. Stuart, Larissa C. Wintle, and Peter J. Leedman   13. Angiogenesis Analysis by in vitro Co-culture Assays in Transwell Chambers in Ovarian Cancer Ali Flores-Pérez, Dolores Gallardo Rincón, Erika Ruiz-García, Raquel Echavarria, Laurence A. Marchat, Elizbeth Álvarez-Sánchez,  and César López-Camarillo   14. Application of Individual qPCR Performance Parameters for Quality Control of Circulating microRNA Data Anna Brunet-Vega, María Elisa Quílez, María José Ramírez-Lázaro, and Sergio Lario   15. Construction of Multi-potent microRNA Sponge and its Functional Evaluation Suhwan Chang   16. microRNA Sequencing Data Analysis Toolkits Wei Wu

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493974337
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 5928 gr
• Formato: Copertina rigida
• Illustration Notes: XI, 222 p. 21 illus., 17 illus. in color.
• Pagine Arabe: 222
• Pagine Romane: xi