Basic and Advanced Laboratory Techniques in Histopathology and Cytology

TRAMA

The second edition of this well-received book provides detailed information on the basic and advanced laboratory techniques in histopathology and cytology. It offers clear guidance on the principles and techniques of routine and special laboratory techniques. It also covers advanced laboratory techniques such as immunocytochemistry, flow cytometry, liquid-based cytology, polymerase chain reactions, tissue microarray, molecular technology, etc.The book's second edition covers several important recent topics with many new chapters, such as liquid biopsy, artificial neural network, digital pathology, and next-generation sequencing.Each chapter elucidates basic principle, practical methods, troubleshooting, and clinical applications of the technique. It includes multiple colored line drawings, microphotographs, and tables to illustrate each technique. The book is a helpful guide to the post-graduate students and fellows in pathology, practicing pathologists, as well as laboratory technicians, and research students.

SOMMARIO

Part I: Basic Laboratory Techniques in Histopathology LaboratoryChapter 1: Fixation of histology samples: Principles, methods and types of fixatives Chapter 2:  Processing of tissue in histopathology laboratoryChapter 3: Embedding of tissue in histopathologyChapter 4: Decalcification of bony and hard tissue for histopathology processingChapter 5: Tissue Microtomy: principle and procedureChapter 6: Frozen section: Principle and procedureChapter 7: Staining principle and general procedure of staining of the tissueChapter 8: Haematoxylin and Eosin stain of the tissue sectionChapter 9: Special Stains for the carbohydrate, protein, lipid, nucleic acid and pigmentsChapter 10: Connective tissue stain: Principle and procedureChapter 11: Amyloid stainingChapter 12: Stains for the Microbial OrganismsPart II: Basic Laboratory Techniques in Cytology LaboratoryChapter 13: Cytology sample procurement, fixation and processingChapter 14: Routine staining in cytology laboratoryChapter 15: Basic technique of fine needle aspiration cytologyPart III: Advanced Techniques in Histology and Cytology LaboratoriesChapter 16: Immunocytochemistry in histology and cytologyChapter 17: Flow cytometry: Basic principles, procedure and applications in pathologyChapter 18: Digital pathology and whole slide imaging Chapter 19: Automation and liquid based cytology in cytology sampleChapter 20: Polymerase chain reaction: Principle, technique and applications in pathology Chapter 21: Fluorescent in situ hybridisation techniques in pathology: Principle, technique and applicationsChapter 22: Tissue microarray in pathology: Principal, technique and applications  Chapter 23: Sanger sequencing and next generation gene sequencing: Basic principles and applications in pathologyChapter 24: Liquid biopsy: Basic principles, techniques and applications Chapter 25: Artificial neural network in Pathology: Basic principles and applicationsPart IV: Microscopy, quality control and laboratory organizationChapter 26: Light microscope and other different microscopesChapter 27: Fluorescence and confocal microscope: Basic principles and applications in pathologyChapter 28: Electron microscopy: Principle, components, optics and specimen processingChapter 29: Quality control and laboratory organization Chapter 30: Laboratory safety and laboratory waste disposalChapter 31:  Multiple choice questions

AUTORE

Pranab Dey is professor in department of cytology and gynecologic pathology at Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India. Professor Dey completed M.D. (Pathology) from PGIMER and FRCPath (Cytopathology) from Royal College of Pathologist, London, UK. Professor Dey has conducted many research projects and has pioneered works on DNA flow cytometry, image morphometry, mono-layered cytology and cytomorphologic findings of various lesions on cytology smears. Professor Dey has more than 35 years of experience in reporting, teaching and research as a consultant. He is a well published author in pathology and a member of various societies. He has more than 450 research paper publications in peer-reviewed journals. He has authored 12 books in cytology and gynecologic pathology. Dr Dey is included in the list of top 2% scientists in the world.

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9789811966156
• Dimensioni: 254 x 178 mm Ø 979 gr
• Formato: Copertina rigida
• Illustration Notes: XXIX, 340 p. 216 illus., 212 illus. in color.
• Pagine Arabe: 340
• Pagine Romane: xxix