Axon Growth and Regeneration

TRAMA

Axon Growth and Regeneration: Methods and Protocols brings together a diverse set of techniques for the study of the mechanisms underlying central nervous system axon growth, consequently providing a resource that will aid in the development of repair strategies. After an introductory section, this detailed volume continues with sections focusing on axon growth in vitro, providing a range of protocols that can be used to examine intracellular signalling pathways, axonal responses to extracellular factors and methods for quantifying outgrowth. The next section provides protocols for inducing experimental injury in vivo as well as some highly promising protocols for promoting regeneration, which segues into the final section highlighting a series of protocols that can be used to monitor the extent of axon regeneration in vivo, ranging from tract tracing to in vivo imaging and functional recovery. As a book in the Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.Practical and reliable, Axon Growth and Regeneration: Methods and Protocols aims to serve researchers studying axon regeneration with a significant set of diverse tools, vital for moving on to the next generation of exciting new discoveries in the field.

SOMMARIO

Part I: Review 1. Axon Regeneration: What Needs to Be Overcome?            Andrew J. Murray Part II: Monitoring Intracellular Pathways in Growth Cones 2. Analysis of Calcium Signals in Steering Neuronal Growth Cones In Vitro            Hiroki Akiyama and Hiroyuki Kamiguchi 3. The Use of Fluorescence Resonance Energy Transfer (FRET) to Measure Axon Growth and Guidance Related Intracellular Signaling in Live Dorsal Root Ganglia Neuronal Growth Cones            Steven J. Tucker Part III: Assessing Axonal Responses to Extracellular Factors In Vitro 4. Neurite Outgrowth and Growth Cone Collapse Assays to Assess Neuronal Responses to Extracellular Cues            Andrew Kaplan, Ricardo Sanz, Gino B. Ferraro, Ricardo Alchini, and Alyson E. Fournier 5. Quantitative Assessment of Neurite Outgrowth in Mouse Retinal Explants            Tom Buyens, Djoere Gaublomme, Inge Van Hove, Lies De Groef, and Lieve Moons 6. Growth Cone Collapse Assay            Geoffrey M.W. Cook, Prem Jareonsettasin, and Roger J. Keynes 7. Axon Length Quantification Microfluidic Culture Platform for Growth and Regeneration Study            Jaewon Park, Sunja Kim, Jianrong Li, and Arum Han 8. Organotypic Slice Co-Culture Systems to Study Axon Regeneration in the Dopaminergic System Ex Vivo            Claudia Heine and Heike Franke 9. Monitoring Neuron and Astrocyte Interactions with a 3D Cell Culture System            James B. Phillips Part IV: Axon Injury and Regeneration In Vivo 10. Targeting Inhibitory Chondroitin Sulphate Proteoglycans to Promote Plasticity After Injury            Jessica C.F. Kwok, Janosch P. Heller, Rong-Rong Zhao, and James W. Fawcett 11. Polymeric Biomaterials for Nerve Regeneration: Fabrication and Implantation of a Biodegradable Nerve Guide            Wesley N. Sivak, Jacqueline M. Bliley, and Kacey G. Marra 12. A Highly Reproducible Mouse Model of Compression Spinal Cord Injury            Suelen Adriani Marques, Fernanda Martins de  Almeida, Klauss Mostacada, and Ana Maria Blanco Martinez 13. Using Templated Agarose Scaffolds to Promote Axon Regeneration Through Sites of Spinal Cord Injury            Jacob Koffler, Ramsey F. Samara, and Ephron S. Rosenzweig 14. In Vivo Electroporation of Adult Mouse Sensory Neurons for Studying Peripheral Axon Regeneration            Saijilafu, Bo-Yin Zhang, and Feng-Quan Zhou Part V: Assessing the Extent of Axon Regeneration 15. Assessing Motor Outcome and Functional Recovery Following Nerve Injury            Ronald Deumens, Claudia Marinangeli, Ahmet Bozkurt, and Gary Anthony Brook 16. The Use of an Adeno-Associated Viral Vector for Efficient Bicistronic Expression of Two Genes in the Central Nervous System            Thomas Haynes Hutson, Claudia Kathe, Sean Christopher Menezes, Marie-Claire Rooney, Hansruedi Bueler, and Lawrence David Falcon Moon 17. Application of Tissue Clearing and Light Sheet Fluorescence Microscopy to Assess Optic Nerve Regeneration in Unsectioned Tissues            Xueting Luo, Benjamin Yungher, and Kevin K. Park 18. Time-Lapse In Vivo Imaging of Dorsal Root Nerve Regeneration in Mice            Andrew Skuba, Meredith Ann Manire, Hyukmin Kim, Seung Baek Han, and Young-Jin Son 19. Using Manganese-Enhanced MRI to Assess Optic Nerve Regeneration            Ioanna Sandvig and Axel Sandvig

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493907762
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 7117 gr
• Formato: Copertina rigida
• Illustration Notes: XI, 253 p. 66 illus., 53 illus. in color.
• Pagine Arabe: 253
• Pagine Romane: xi