Antibiotic Resistance Protocols

TRAMA

This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response.  New and updated chapters cover techniques from the microscopic scale to whole animal models. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Antibiotic Resistance Protocols, Third Edition aims to ensure successful results in the further study of this vital field.

SOMMARIO

1.  Methods for Measuring the Production of Quorum Sensing Signal Molecules Manuel Alcalde-Rico and José Luis Martínez 2.  Construction and Use of Staphylococcus aureus Strains to Study Within-Host Infection Dynamics  Gareth McVicker, Tomasz K. Prajsnar, and Simon J. Foster 3.  Method for Detecting and Studying Genome-wide Mutations in Single Living Cells in Real-time  Marina Elez, Lydia Robert, and Ivan Matic 4.  Detecting Phenotypically Resistant Mycobacterium tuberculosis using Wavelength Modulated Raman Spectroscopy Vincent O. Baron, Mingzhou Chen, Simon O. Clark, Ann Williams, and Stephen H. Gillespie 5. A Flow Cytometry Method for Assessing M. tuberculosis Responses to Antibiotics   Charlotte L. Hendon-Dunn, Stephen R. Thomas, Stephen C. Taylor, and Joanna Bacon 6.  Application of Continuous Culture for Assessing Antibiotic Activity against Mycobacterium tuberculosis  Charlotte Hendon-Dunn, Saba Anwar, Christopher Burton, and Joanna Bacon 7.  Real-Time Digital Bright Field Technology for Rapid Antibiotic Susceptibility Testing   Chiara Canali, Erik Spillum, Martin Valvik, Niels Agersnap, and Tom Olesen 8.  Enhanced Methodologies for Detecting Phenotypic Resistance in Mycobacteria  Robert J H Hammond, Vincent Baron, Sam Lipworth, and Stephen H. Gillespie 9.  Methods to Determine Mutational Trajectories after Experimental Evolution of Antibiotic Resistance Douglas Huseby and Diarmaid Hughes 10.  Selection of ESBL-producing E. coli in a Mouse Intestinal Colonization Model Frederik Boëtius Hertz, Karen Leth Nielsen, and Niels Frimodt-Møller 11.  Transcriptional Profiling Mycobacterium tuberculosis from Patient Sputa Leticia Muraro-Wildner, Katherine A Gould, and Simon J Waddell 12. Direct Agarose Gel Probing, Pulsed Field Gel Electrophoresis, Plasmid detection, Genomic Location, and Resistance Gene Mapping  Mark A Toleman 13. Using RT qPCR for Quantifying Mycobacteria marinum from In-vitro and In-vivo Samples Han Xaio and Stephen H. Gillespie 14. Use of Larval Zebrafish Model to Study Within-Host Infection Dynamics  Tomasz K. Prajsnar, Gareth McVicker, Alexander Williams, Stephen A. Renshaw, and Simon J. Foster  15.  A Method to Evaluate Persistent Bacteria in vitro and in the Cornell Model of Tuberculosis Yanmin Hu and Anthony Coates

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781493985340
• Collana: Methods in Molecular Biology
• Dimensioni: 254 x 178 mm Ø 454 gr
• Formato: Brossura
• Illustration Notes: X, 168 p. 32 illus., 5 illus. in color.
• Pagine Arabe: 168
• Pagine Romane: x