Adhesion Protein Protocols

SOMMARIO

Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells.- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation.- Analysis of Neutrophil Chemotaxis.- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells.- Biochemical Purification of Pseudopodia from Migratory Cells.- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture.- Quantitative Analyses of Cell Adhesion Strength.- Using RNA Interference to Knock Down the Adhesion Protein TES.- Assaying Calpain Activity.- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray.- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells.- Bioinformatic Analysis of Adhesion Proteins.- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching.- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture.- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.

ALTRE INFORMAZIONI

• Condizione: Nuovo
• ISBN: 9781588295330
• Collana: Methods in Molecular Biology
• Dimensioni: 235 x 155 mm
• Formato: Copertina rigida
• Illustration Notes: X, 234 p. 54 illus., 2 illus. in color.
• Pagine Arabe: 234
• Pagine Romane: x