Transgenic Plants Methods and Protocols

This book provides thorough coverage of transgenic plants with methods on plant transformation, biotechnological application of transgenic plants, and future developments. Chapters are grouped into sections focusing on transformation model and crop plants, genome engineering, and transgenic event characterization. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Transgenic Plants: Methods and Protocols aims to broaden the utility for readers, provide additional references for further understanding, and present the technology’s potential for solving some of our most urgent global challenges in food security.

1.       Repurposing macromolecule delivery tools for plant genetic modification in the era of precision genome engineering

2.       The use of an automated platform to assemble multigenic constructs for plant transformation

4.       Setaria viridis as a model plant for functional genomic studies in C4 crops

5.       Transient transformation using particle bombardment for gene expression analysis

6.       Maize transformation using the morphogenic genes babyboom and wuschel2

7.       Efficient and fast production of transgenic rice plants by agrobacterium-mediated transformation

8.       Protocol for Agrobacterium-mediated transformation and transgenic plant production of switchgrass

9.       Biolistic transformation of wheat

10.   Mesophyll protoplasts and PEG-mediated transfections: Transient assays and generation of stable transgenic canola plants

11.   A Unified Agrobacterium-mediated transformation protocol for alfalfa (Medicago sativa l.) and medicago truncatula

12.   Poplar transformation

13.   The genetic transformation of sweet orange (Citrus sinensis L. Osbeck) for enhanced resistance to citrus canker

14.   Genetic modification of grapevine embryogenic culture

15.   Agrobacterium-mediated transformation of Solanum tuberosum L., potato

16.   Agrobacterium tumefaciens-mediated transformation of tomato

17.   DNA break repair in plants and its application for genome engineering

18.   Gene stacking in plants through the application of site-specific recombination and nuclease activity

19.   CRISPR/Cas9 for mutagenesis in rice

20.   Plant biotechnology applications of zinc fingers technology

21.   Overview of biotechnology-derived herbicide tolerance and insect resistance traits in plant agriculture

23.   Transgenic and genome editing approaches for modifying plant oils

24.   Summary of molecular analysis methods for characterizing transgenic events

25.   Detection of transgenic proteins by immunoassays

26.   Systematic evaluation of field crop performance using modern phenotyping tools and techniques