Peptide Antibodies Methods and Protocols

This detailed new edition explores current methods for the production and use of peptide antibodies. The book delves into various aspects of peptide synthesis and analysis, peptide-carrier conjugation, epitope and paratope prediction and identification, as well as improved assays and other uses of peptide antibodies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and up-to-date, Peptide Antibodies: Methods and Protocols, Second Edition serves as an ideal reference for researchers exploring this vital and expansive area of study.

Peptide Antibodies: Current Status.- B-Cell Epitope Prediction for Antipeptide Paratopes with the HAPTIC2/HEPTAD User Toolkit (HUT).- Fmoc Solid-Phase Peptide Synthesis.- Peptide-Carrier Conjugation.- Solid Phase Peptide Carrier Conjugation.- Analysis of Polypeptides by Amino Acid Analysis.- Characterization of Synthetic Peptides by Mass Spectrometry.- Interpretation of Tandem Mass Spectrometry (MS-MS) Spectra for Peptide Analysis.- Cyclic Peptide Conjugate Vaccines and Physically Mixed Cyclic Peptide Vaccines for Subcutaneous Immunization.- Polyclonal Peptide Antisera.- Production of Antibodies to Peptide Targets Using Hybridoma Technology.- Affinity Chromatography for Anti-Glucosylated Adhesin Antibodies Purification, Depletion of Non-Specific Anti-Protein Antibodies and Antibody Recovery with Unconventional Elution Solutions.- Identification and Validation of Peptides Specifying SARS-CoV-2 B-Cell Epitopes Eliciting Neutralizing Antibodies.- Characterization of Peptide Antibodies by Epitope Mapping Using Resin-Bound and Soluble Peptides.- Structural Characterization of Peptide Antibodies.- An Analytical Protocol for Detecting Antibody Titer Levels in Serum/Saliva by Indirect Enzyme-Linked Immunosorbent Assay (ELISA).- Enzyme-Linked Immunosorbent Assay for Antibodies Against the Tumor-Associated Antigen-Derived Cytotoxic T Lymphocyte Epitope.- Assessment of the Antigen-Binding Capacity and Separation of Extracellular Vesicles Coated with Antigen-Specific Antibody Light Chains.- Sequential Double Immunoblotting with Peptide Antibodies.- Cytochemical and Histochemical Staining with Peptide Antibodies.