Esophageal Adenocarcinoma Methods and Protocols

This vital collection provides a unique set of techniques to explore the clinical, pathological, and research aspects of the management of patients with esophageal adenocarcinoma. Beginning with an introduction to the basic features of esophageal adenocarcinoma, the book continues with clinical protocols for the management of the cancer, pathological methods for management of the patients and research, as well as protocols for molecular research, which could aid researchers in furthering our understanding of pathogenesis as well as in identifying new targets for the treatment and prevention of adenocarcinoma. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. 

1. Introduction: Esophageal Adenocarcinoma:  Updates of Current Status

            Alfred K. Lam

 

2. Radiotherapy for Esophageal Adenocarcinoma

            Dora L.W. Kwong

 

3. Chemotherapy for Esophageal Adenocarcinoma

            Ka-On Lam and Dora L.W. Kwong

 

4. Surgical Protocol for Esophageal Adenocarcinoma

            Simon Law

 

5. Target Therapy for Esophageal Adenocarcinoma

            Ka-On Lam and Dora L.W. Kwong

 

6. Histopathological Assessment for Esophageal Adenocarcinoma

            Alfred K. Lam

 

7. Macroscopic Assessment and Cut Up of Endoscopic Resection Specimens for Early Esophageal Glandular Malignancies

            Benjamin M. Allanson and Marian P. Kumarasinghe

 

8. Processing of Surgical Specimen (Esophagogastrectomy) for Esophageal Adenocarcinoma

            Benjamin M. Allanson and Marian P. Kumarasinghe

 

9. Application of Pathological Staging in Esophageal Adenocarcinoma

            Alfred K. Lam

 

10. Application of Tissue Microarray in Esophageal Adenocarcinoma

            Nassim Saremi and Alfred K. Lam

 

11. Predictive Marker: HER2 in Esophageal Adenocarcinoma

            Duminda Subasinghe, Nathan Acott, and Marian P. Kumarasinghe

 

12. Whole-Slide Imaging for Esophageal Adenocarcinoma

            Alfred K. Lam and Melissa Leung

 

13. Animal Model: Reflux Models in Esophageal Adenocarcinoma

            Takahiro Masuda and Sumeet K. Mittal

 

14. Animal Model: Xenograft Mouse Models in Esophageal Adenocarcinoma

            Md Sazzad Hassan and Urs von Holzen

 

15. Identification of Cancer Stem Cells in Esophageal Adenocarcinoma

            Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

 

16. Circulatory Tumor Cells in Esophageal Adenocarcinoma

            Vinod Gopalan and Alfred K. Lam

 

17. Liquid Biopsy for Investigation of Cancer DNA in Esophageal Adenocarcinoma: Cell-Free Plasma DNA and Exosome-Associated DNA

            Robert A. Smith and Alfred K. Lam

 

18. Somatic DNA Copy Number Alterations Detection for Esophageal Adenocarcinoma Using Digital Polymerase Chain Reaction

            Katherine T.W. Lee, Vinod Gopalan, and Alfred K. Lam

 

19. Targeted Single Gene Mutation in Esophageal Adenocarcinoma

            Katherine T.W. Lee, Robert A. Smith , Vinod Gopalan, and Alfred K. Lam

 

20. DNA Genome Sequencing in Esophageal Adenocarcinoma

            Suja Pillai, Vinod Gopalan, and Alfred K. Lam

 

21. Epigenetics: DNA Methylation Analysis in Esophageal Adenocarcinoma

            Farhadul Islam, Johnny C. Tang, Vinod Gopalan, and Alfred K. Lam

 

22. Detection and Quantification of MicroRNAs in Esophageal Adenocarcinoma

            Moien Amin, Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

 

23. RNA Interference-Mediated Gene Silencing in Esophageal Adenocarcinoma

            Farhadul Islam, Vinod Gopalan, and Alfred K. Lam

 

24. Proteomic Protocol in Esophageal Adenocarcinoma

            Peter Kelly